| The isolation and characterization of a novel telomerase immortalized first trimester trophoblast cell line, Swan 71. | |
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MedLine Citation:
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PMID: 19766308 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Studies using first trimester trophoblast cells may be limited by the inability to obtain patient samples and/or adequate cell numbers. First trimester trophoblast cell lines have been generated by SV40 transformation or similar methods, however, this approach is known to induce phenotypic and karyotypic abnormalities. The introduction of telomerase has been proposed to be a viable alternative for the immortalization of primary human cells. To investigate whether telomerase-induced immortalization might be a more feasible approach for the generation of first trimester trophoblast cell lines, we isolated primary trophoblast cells from a 7-week normal placenta and infected the cells with human telomerase reverse transcriptase (hTERT), the catalytic subunit of telomerase. Although this hTERT-infected first trimester trophoblast cell line, which we have named Swan 71, has been propagated for more than 100 passages, it still has attributes that are characteristic of primary first trimester trophoblast cells. The Swan 71 cells are positive for the expression of cytokeratin 7, vimentin and HLA-G, but do not express CD45, CD68 or the Fibroblast Specific Antigen (FSA), CD90/Thy-1. In addition, we also demonstrated that the Swan 71 cells secrete fetal fibronectin (FFN) as well as low levels of human Chorionic Gonadotrophin (hCG). Moreover, the Swan 71 cells exhibit a cytokine and growth factor profile that is similar to primary trophoblast cells and are resistant to Fas, but not TNF-alpha-induced apoptosis. This suggests that the Swan 71 cells may represent a valuable model for future in vitro trophoblast studies. |
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Authors:
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S L Straszewski-Chavez; V M Abrahams; A B Alvero; P B Aldo; Y Ma; S Guller; R Romero; G Mor |
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Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Intramural Date: 2009-09-18 |
Journal Detail:
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Title: Placenta Volume: 30 ISSN: 1532-3102 ISO Abbreviation: Placenta Publication Date: 2009 Nov |
Date Detail:
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Created Date: 2009-11-02 Completed Date: 2010-01-07 Revised Date: 2011-05-30 |
Medline Journal Info:
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Nlm Unique ID: 8006349 Medline TA: Placenta Country: England |
Other Details:
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Languages: eng Pagination: 939-48 Citation Subset: IM |
Affiliation:
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Department of Molecular, Cellular and Developmental Biology, Yale University, New Haven, CT, USA. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Antigens, CD
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biosynthesis Antigens, CD45 / biosynthesis Antigens, Differentiation, Myelomonocytic / biosynthesis Antigens, Thy-1 / biosynthesis Apoptosis / drug effects Cell Line* Chorionic Gonadotropin / secretion Cytokines / biosynthesis Female Fibronectins / secretion HLA Antigens / biosynthesis Histocompatibility Antigens Class I / biosynthesis Humans Intercellular Signaling Peptides and Proteins / biosynthesis Keratin-7 / biosynthesis Pregnancy Pregnancy Trimester, First / genetics* Telomerase / metabolism* Trophoblasts / cytology*, metabolism Vimentin / biosynthesis |
| Grant Support | |
ID/Acronym/Agency:
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P01 HD054713-01A2/HD/NICHD NIH HHS; P01 HD054713-01A25182/HD/NICHD NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Antigens, CD; 0/Antigens, Differentiation, Myelomonocytic; 0/Antigens, Thy-1; 0/CD68 antigen, human; 0/Chorionic Gonadotropin; 0/Cytokines; 0/FFN protein, human; 0/Fibronectins; 0/HLA Antigens; 0/HLA-G antigen; 0/Histocompatibility Antigens Class I; 0/Intercellular Signaling Peptides and Proteins; 0/Keratin-7; 0/Vimentin; EC 2.7.7.49/Telomerase; EC 3.1.3.48/Antigens, CD45; EC 3.1.3.48/PTPRC protein, human |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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