Document Detail


The isolation and characterization of a glycoprotein from human thoracic aorta.
MedLine Citation:
PMID:  5696869     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
1. A glycoprotein extracted by cold alkali from the walls of human aorta was purified by chromatography on DEAE-cellulose. 2. The compound was electrophoretically homogeneous and essentially so by chromatography on DEAE-cellulose. Ultracentrifugal examination revealed two components, and it is suggested that the faster-sedimenting component represents an aggregated form of the glycoprotein. 3. Glycoprotein preparations contained approx. 8% of carbohydrate. Digestion with Pronase yielded a glycopeptide fraction containing all the carbohydrate of the glycoprotein. The glycopeptide, of molecular weight about 7800, contained sialic acid, galactose, mannose, fucose and hexosamine in the approximate molar proportions 5:10:5:2:11. Sialic acid was terminal with respect to the polysaccharide chains. 4. Both elastase and elastomucoproteinases exhibited proteolytic activity towards the glycoprotein. Studies by other investigators have led to the conclusion that elastomucoproteinases attack protein-carbohydrate complexes occurring in intimate association with elastin in aorta and other tissues, and it is suggested that the glycoprotein may be identified with one of these compounds.
Authors:
M J Barnes; S M Partridge
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  The Biochemical journal     Volume:  109     ISSN:  0264-6021     ISO Abbreviation:  Biochem. J.     Publication Date:  1968 Oct 
Date Detail:
Created Date:  1969-01-05     Completed Date:  1969-01-05     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  2984726R     Medline TA:  Biochem J     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  883-96     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Adolescent
Adult
Age Factors
Aged
Aorta, Thoracic / analysis*
Carbohydrates / analysis
Cellulose
Centrifugation
Child
Child, Preschool
Chromatography, Ion Exchange
Elastin
Electrophoresis
Endopeptidases
Fucose / analysis
Galactose / analysis
Glycoproteins / isolation & purification*
Hexosamines / analysis
Humans
Infant
Infant, Newborn
Mannose / analysis
Middle Aged
Molecular Weight
Neuraminic Acids / analysis
Pancreatic Elastase
Polysaccharides
Chemical
Reg. No./Substance:
0/Carbohydrates; 0/Glycoproteins; 0/Hexosamines; 0/Neuraminic Acids; 0/Polysaccharides; 26566-61-0/Galactose; 31103-86-3/Mannose; 3713-31-3/Fucose; 9004-34-6/Cellulose; 9007-58-3/Elastin; EC 3.4.-/Endopeptidases; EC 3.4.21.36/Pancreatic Elastase
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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