Document Detail

The isolation of HTC variant cells which can replicate in butyrate. Changes in histone acetylation and tyrosine aminotransferase induction.
MedLine Citation:
PMID:  2860113     Owner:  NLM     Status:  MEDLINE    
We have obtained a number of variant HTC cells which are capable of vigorous replication in the presence of 6 mM sodium butyrate. These cells show characteristic changes in histone acetylation. H2A/H2B are no longer modified and the turnover of histones H3/H4 acetate is about 4-fold greater than in control HTC cells at the same butyrate concentration. Histone deposition continues successfully even though histones H3/H4 become hyperacetylated upon association with the chromatin. Prompt deacetylation of new histones does not appear to be a prerequisite for successful deposition processes. Initial enzymatic studies indicate that not only do the butyrate-resistant cells show an increased deacetylase activity (on a per cell basis), but also the enzyme is less sensitive to sodium butyrate under in vitro assay conditions. In contrast to control HTC cells in 6 mM butyrate in which dexamethasone induction of tyrosine aminotransferase is inhibited, the butyrate-resistant variant cells are capable of tyrosine aminotransferase induction even in the presence of butyrate. The implications of these observations are discussed.
R Chalkley; A Shires
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  The Journal of biological chemistry     Volume:  260     ISSN:  0021-9258     ISO Abbreviation:  J. Biol. Chem.     Publication Date:  1985 Jun 
Date Detail:
Created Date:  1985-07-25     Completed Date:  1985-07-25     Revised Date:  2009-11-19    
Medline Journal Info:
Nlm Unique ID:  2985121R     Medline TA:  J Biol Chem     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  7698-704     Citation Subset:  IM    
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MeSH Terms
Butyrates / pharmacology*
Butyric Acid
Butyric Acids / metabolism,  pharmacology*
Cell Line
Cell Nucleus / drug effects,  enzymology
Dexamethasone / pharmacology
Drug Resistance
Enzyme Induction
Genetic Variation*
Histone Deacetylases / metabolism
Histones / isolation & purification,  metabolism*
Liver Neoplasms, Experimental / metabolism*
Tyrosine Transaminase / biosynthesis*
Grant Support
Reg. No./Substance:
0/Butyrates; 0/Butyric Acids; 0/Histones; 107-92-6/Butyric Acid; 50-02-2/Dexamethasone; EC Transaminase; EC Deacetylases

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