| The isolation of 6-alpha-hydroxyoestrone from the urine of pregnant women. | |
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MedLine Citation:
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PMID: 5966276 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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1. In view of previous experiments in vitro and in vivo, in which 6-hydroxylation of phenolic steroids by various tissues was demonstrated, an attempt was made to isolate 6-hydroxy oestrogens from the urine of pregnant women. 2. During this work it was found that free 6alpha- as well as 6beta-hydroxylated oestrogens were extremely unstable under acidic conditions (pH <3); it was therefore necessary to establish experimental conditions under which no rearrangement of the hydroxyl group would take place. 3. By avoiding acid steps during the experimental procedures, a ketonic-phenolic fraction was obtained from enzymically hydrolysed late-pregnancy urine that was subjected to paper chromatography in various systems. 4. By using similar methods on a large scale and partition chromatography on Celite columns, a crystalline material was obtained from 1001. of late-pregnancy urine that was identified as 6alpha-hydroxyoestrone by various chemical reactions and by infrared spectroscopy. |
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Authors:
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R Knuppen; O Haupt; H Breuer |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: The Biochemical journal Volume: 101 ISSN: 0264-6021 ISO Abbreviation: Biochem. J. Publication Date: 1966 Nov |
Date Detail:
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Created Date: 1967-08-03 Completed Date: 1967-08-03 Revised Date: 2009-11-18 |
Medline Journal Info:
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Nlm Unique ID: 2984726R Medline TA: Biochem J Country: ENGLAND |
Other Details:
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Languages: eng Pagination: 397-401 Citation Subset: IM |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Chromatography, Paper Estrogens / urine* Female Humans Hydrochloric Acid Pregnancy* Spectrophotometry |
| Chemical | |
Reg. No./Substance:
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0/Estrogens; 7647-01-0/Hydrochloric Acid |
| Comments/Corrections | |
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