| An isolated perfused rat lung preparation for the study of aerosolized drug deposition and absorption. | |
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MedLine Citation:
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PMID: 3958927 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The isolated, perfused rat lung preparation was modified to allow characterized solid aerosol delivery. Deposition and airway-to-perfusate transfer of disodium fluorescein from 3-4 micron dae solid aerosols were studied under different ventilatory regimes. The lungs inhaled from an aerosol stream of constant concentration via a tracheal cannula. Air displacement from a sealed artificial thorax housing the lungs provided the driving force for inhalation. The lungs were suspended in a physiologically normal position and both left and right sides of the heart were cannulated for constant rate perfusate flow. Fractional deposition was inversely proportional to respiratory frequency implying that sedimentation was the primary deposition mechanism. Increasing tidal volumes similarly enhanced the ratio of amount deposited/amount administered. Fluorescein transfer to the perfusate occurred from the lung regions containing intact vasculature, was apparent first-order, and independent of perfusate flow. The average rate constant for transfer was 0.057 +/- 0.02 min-1 (t1/2 = 12.2 +/- 4.2 min-1). The ratio of transferable amount/amount deposited appeared to indicate the depth of aerosol penetration. This increased at high respiratory frequency and tidal volume, while decreasing with increasing aerosol particle size. Potential applications of the model are discussed in the light of these results. |
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Authors:
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P R Byron; N S Roberts; A R Clark |
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Publication Detail:
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Type: In Vitro; Journal Article |
Journal Detail:
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Title: Journal of pharmaceutical sciences Volume: 75 ISSN: 0022-3549 ISO Abbreviation: J Pharm Sci Publication Date: 1986 Feb |
Date Detail:
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Created Date: 1986-05-22 Completed Date: 1986-05-22 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 2985195R Medline TA: J Pharm Sci Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 168-71 Citation Subset: IM |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Absorption Aerosols Animals Fluorescein Fluoresceins / metabolism Lung / metabolism* Male Particle Size Perfusion Pharmaceutical Preparations / metabolism* Rats Rats, Inbred Strains Time Factors |
| Chemical | |
Reg. No./Substance:
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0/Aerosols; 0/Fluoresceins; 0/Pharmaceutical Preparations; 2321-07-5/Fluorescein |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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