Document Detail

The isolated perfused liver response to a 'second hit' of portal endotoxin during severe acute pancreatitis.
MedLine Citation:
PMID:  15985775     Owner:  NLM     Status:  MEDLINE    
BACKGROUND/AIM: During severe acute pancreatitis (AP), the liver may show an exaggerated response to the inflammatory products of gut injury transported in the portal vein. Our aim was to explore liver proinflammatory mediator production after a 'second hit' of portal lipopolysaccharide (LPS) during AP. METHODS: Twenty-four rats underwent one of three 'first-hit' scenarios: (1) severe AP induced by intraductal glycodeoxycholic acid injection and intravenous caerulein infusion, (2) sham laparotomy, or (3) no first intervention. Eighteen hours later, all animals received a 'second hit' of portal LPS in an isolated liver perfusion system. Tumour necrosis factor-alpha (TNF-alpha), interleukin (IL)-1beta, and IL-6 concentrations were measured in portal and systemic serum, and in the perfusate 30 and 90 min after the 'second hit'. Neutrophil activation by the perfusate was assayed using dihydrorhodamine-123 fluorescence. RESULTS: We observed a six-fold increase in IL-6 concentration across the liver during AP. All livers produced TNF-alpha after the portal LPS challenge, but this was not exaggerated by AP. No differential neutrophil activation by the perfusate was seen. CONCLUSION: TNF-alpha, IL-1beta, IL-6 and neutrophil activator production by the isolated perfused liver, in response to a 'second hit' of portal LPS, does not appear to be enhanced during AP.
Damian J Mole; Mark A Taylor; Neil V McFerran; Thomas Diamond
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2005-06-28
Journal Detail:
Title:  Pancreatology : official journal of the International Association of Pancreatology (IAP) ... [et al.]     Volume:  5     ISSN:  1424-3903     ISO Abbreviation:  Pancreatology     Publication Date:  2005  
Date Detail:
Created Date:  2005-08-09     Completed Date:  2005-09-29     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  100966936     Medline TA:  Pancreatology     Country:  Switzerland    
Other Details:
Languages:  eng     Pagination:  475-85     Citation Subset:  IM    
Copyright Information:
Copyright 2005 S. Karger AG, Basel and IAP.
Department of Surgery, Queen's University of Belfast, Belfast, Northern Ireland.
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MeSH Terms
Amylases / blood
Animals, Outbred Strains
Caerulein / pharmacology
Disease Models, Animal
Glycodeoxycholic Acid / pharmacology
Interleukin-6 / metabolism
Lipopolysaccharides / adverse effects*
Liver / drug effects*,  metabolism
Neutrophil Activation / drug effects
Neutrophils / drug effects,  metabolism
Pancreas / drug effects,  pathology
Pancreatitis, Acute Necrotizing / blood,  chemically induced,  pathology*
Rats, Wistar
Respiratory Burst / drug effects
Systemic Inflammatory Response Syndrome / chemically induced,  pathology*
Tumor Necrosis Factor-alpha / metabolism
Reg. No./Substance:
0/Interleukin-6; 0/Lipopolysaccharides; 0/Tumor Necrosis Factor-alpha; 17650-98-5/Caerulein; 360-65-6/Glycodeoxycholic Acid; EC 3.2.1.-/Amylases

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