| An isocratic LC method for the simultaneous determination of vitamins A, C, E and beta-carotene. | |
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MedLine Citation:
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PMID: 10703996 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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An isocratic liquid chromatographic method for the separation and simultaneous determination of retinyl acetate, propionate or palmitate (esters of vitamin A), beta-carotene (pro-vitamin A), ascorbic acid (vitamin C) and DL-alpha-tocopherol (vitamin E) is described. Samples are analysed by means of a reversed-phase column (LiChrospher 100 RP-18), using methanol as mobile phase. The UV Vis detector used was set at a wavelength of 300 nm and switched to 450 nm at 17 min, allowing the determination of beta-carotene. These vitamins were separated within 25 min and the detection limits ranged from 7 (beta-carotene) to 65 ng ml(-1) (ascorbic acid). |
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Authors:
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M G Paulo; H M Marques; J A Morais; A J Almeida |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Journal of pharmaceutical and biomedical analysis Volume: 21 ISSN: 0731-7085 ISO Abbreviation: J Pharm Biomed Anal Publication Date: 1999 Nov |
Date Detail:
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Created Date: 2000-03-13 Completed Date: 2000-03-13 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 8309336 Medline TA: J Pharm Biomed Anal Country: ENGLAND |
Other Details:
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Languages: eng Pagination: 399-406 Citation Subset: IM |
Affiliation:
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Faculdade de Farmácia da Universidade de Lisboa, Unidade de Ciências e Tecnologia Farmacêuticas, Portugal. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Ascorbic Acid
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analysis* Chromatography, High Pressure Liquid / methods* Excipients / chemistry Methanol Regression Analysis Reproducibility of Results Time Factors Vitamin A / analysis* Vitamin E / analysis* beta Carotene / analysis* |
| Chemical | |
Reg. No./Substance:
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0/Excipients; 11103-57-4/Vitamin A; 1406-18-4/Vitamin E; 50-81-7/Ascorbic Acid; 67-56-1/Methanol; 7235-40-7/beta Carotene |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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