| The involvement of two cdc2-related kinases (CRKs) in Trypanosoma brucei cell cycle regulation and the distinctive stage-specific phenotypes caused by CRK3 depletion. | |
| | |
MedLine Citation:
|
PMID: 15010459 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
Cyclin-dependent protein kinases are among the key regulators of eukaryotic cell cycle progression. Potential functions of the five cdc2-related kinases (CRK) in Trypanosoma brucei were analyzed using the RNA interference (RNA(i)) technique. In both the procyclic and bloodstream forms of T. brucei, CRK1 is apparently involved in controlling the G(1)/S transition, whereas CRK3 plays an important role in catalyzing cells across the G(2)/M junction. A knockdown of CRK1 caused accumulation of cells in the G(1) phase without apparent phenotypic change, whereas depletion of CRK3 enriched cells of both forms in the G(2)/M phase. However, two distinctive phenotypes were observed between the CRK3-deficient procyclic and bloodstream forms. The procyclic form has a majority of the cells containing a single enlarged nucleus plus one kinetoplast. There is also an enhanced population of anucleated cells, each containing a single kinetoplast known as the zoids (0N1K). The CRK3-depleted bloodstream form has an increased number of one nucleus-two kinetoplast cells (1N2K) and a small population containing aggregated multiple nuclei and multiple kinetoplasts. Apparently, these two forms have different mechanisms in cell cycle regulation. Although the procyclic form can be driven into cytokinesis and cell division by kinetoplast segregation without a completed mitosis, the bloodstream form cannot enter cytokinesis under the same condition. Instead, it keeps going through another G(1) phase and enters a new S phase resulting in an aggregate of multiple nuclei and multiple kinetoplasts in an undivided cell. The different leakiness in cell cycle regulation between two stage-specific forms of an organism provides an interesting and useful model for further understanding the evolution of cell cycle control among the eukaryotes. |
| | |
Authors:
|
Xiaoming Tu; Ching C Wang |
Related Documents
:
|
284329 - Caulobacter crescentus nucleoid: analysis of sedimentation behavior and protein composi... 12527899 - Identification of a novel cell cycle regulated gene, hurp, overexpressed in human hepat... 1193599 - Human triploid cell strain. phenotype on cellular level. 19048389 - Gadd45-alpha and gadd45-gamma utilize p38 and jnk signaling pathways to induce cell cyc... 15087469 - Foxo1a can alter cell cycle progression by regulating the nuclear localization of p27ki... 17466069 - The lin-35/rb and rnai pathways cooperate to regulate a key cell cycle transition in c.... 21946519 - Novel role of wip1 in p53-mediated cell homeostasis under a non-stress condition. 6100019 - Identification and possible function of pro-opiomelanocortin-derived peptides in the te... 12191009 - Contractile actin expression in torn human menisci. |
Publication Detail:
|
Type: Journal Article; Research Support, U.S. Gov't, P.H.S. Date: 2004-03-08 |
Journal Detail:
|
Title: The Journal of biological chemistry Volume: 279 ISSN: 0021-9258 ISO Abbreviation: J. Biol. Chem. Publication Date: 2004 May |
Date Detail:
|
Created Date: 2004-05-04 Completed Date: 2004-06-15 Revised Date: 2009-11-19 |
Medline Journal Info:
|
Nlm Unique ID: 2985121R Medline TA: J Biol Chem Country: United States |
Other Details:
|
Languages: eng Pagination: 20519-28 Citation Subset: IM |
Affiliation:
|
Department of Pharmaceutical Chemistry, University of California-San Francisco, San Francisco, CA 94143-2280, USA. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Animals Antimetabolites, Antineoplastic / pharmacology CDC2 Protein Kinase / physiology* Cell Cycle Cell Division Cell Nucleus / metabolism Cell Separation Coloring Agents / pharmacology DNA, Complementary / metabolism Flow Cytometry G1 Phase G2 Phase Mitosis Phenotype Protozoan Proteins / physiology* RNA Interference Reverse Transcriptase Polymerase Chain Reaction S Phase Time Factors Transfection Trypanosoma brucei brucei / enzymology* |
| Grant Support | |
ID/Acronym/Agency:
|
R01 AI 2178/AI/NIAID NIH HHS |
| Chemical | |
Reg. No./Substance:
|
0/Antimetabolites, Antineoplastic; 0/Coloring Agents; 0/DNA, Complementary; 0/Protozoan Proteins; EC 2.7.1.37/crk1 protein,Trypanosoma brucei; EC 2.7.10.-/CRK3 kinase, protozoa; EC 2.7.11.22/CDC2 Protein Kinase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: A novel human Cl(-) channel family related to Drosophila flightless locus.
Next Document: Cisplatin-induced post-translational modification of histones H3 and H4.