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The involvement of serotonin metabolism in cigarette smoke-induced oxidative stress in rat lung in vivo.
MedLine Citation:
PMID:  22900927     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
Abstract Recently, we have reported the dysregulation of circulating serotonin (5-hydroxytryptamine, 5-HT) homeostasis in patients with chronic obstructive pulmonary disease (COPD). An increase in metabolism of 5-HT has been reported to induce oxidative stress via monoamine oxidase (MAO)-dependent pathway. The present study aimed at investigating the effect of cigarette smoke exposure on the systemic circulation and local airway 5-HT levels as well as MAO-mediated oxidative pathway using a cigarette smoke-exposed rat model. Male Sprague-Dawley rats (150-200g) were exposed to either sham air or 4% (v/v, smoke/air) cigarette smoke for 1 h daily for 56 consecutive days. Sera, bronchoalveolar larvage (BAL) and lung tissues were collected 24 h after the last exposure. We found a significant reduction in the reduced glutathione (rGSH) and an elevation in advanced oxidation protein products (AOPP), a protein oxidation marker, in the lung of cigarette smoke-exposed group (p<0.05). A significant increase in 5-HT was found in serum (p<0.05), but not in the BAL or lung, after cigarette smoke exposure. MAO-A activity was significantly elevated in the lung of cigarette smoke-exposed group (p<0.05). Furthermore, increased superoxide anion levels were found in lung homogenates of cigarette smoke-exposed rats after incubation with 5-HT (p<0.05), which was positively associated with the increase in MAO-A activity (r = 0.639, p<0.05). Our findings supported the presence of GSH disruption and protein oxidation in the lung after cigarette smoke exposure. The metabolism of 5-HT by MAO-A in the lung enhanced cigarette smoke-induced superoxides, which might contribute to the pathogenesis of COPD.
Authors:
Way Kwok-Wai Lau; Xiang Li; Dave Sze-Chun Yeung; Ka-Ho Chan; Mary Sau-Man Ip; Judith Choi-Wo Mak
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-8-17
Journal Detail:
Title:  Free radical research     Volume:  -     ISSN:  1029-2470     ISO Abbreviation:  Free Radic. Res.     Publication Date:  2012 Aug 
Date Detail:
Created Date:  2012-8-20     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9423872     Medline TA:  Free Radic Res     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
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