| The involvement of protein kinase C in activation-induced cell death in T-cell hybridoma. | |
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MedLine Citation:
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PMID: 1394438 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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T-cell hybridoma activated by a variety of stimuli such as anti-cell surface antigen, notably CD3 and T-cell receptors, and Con A undergoes a cell lysis process called activation-induced cell death (AICD). It was found that the major protein kinase C (PKC) isoform in the 2B4.11 T-cell hybridoma, PKC(alpha), was translocated from the cytosolic to the particulate fraction when these hybridoma cells were induced to die by plastic-adsorbed anti-CD3 antibodies. Inhibitors of protein phosphorylation rescued 2B4.11 cells from AICD as determined by the analysis of cellular metabolism and the proportion of living cells. Furthermore, PKC(alpha) down-regulation by phorbol ester treatment abolished AICD, and the degree of PKC down-regulation correlated well with the degree of AICD abolishment, suggesting that PKC activation represents an essential step in the molecular mechanisms underlying AICD in this T-cell hybridoma. |
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Authors:
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L W Jin; K Inaba; T Saitoh |
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Publication Detail:
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Type: Journal Article; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: Cellular immunology Volume: 144 ISSN: 0008-8749 ISO Abbreviation: Cell. Immunol. Publication Date: 1992 Oct |
Date Detail:
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Created Date: 1992-10-28 Completed Date: 1992-10-28 Revised Date: 2007-11-15 |
Medline Journal Info:
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Nlm Unique ID: 1246405 Medline TA: Cell Immunol Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 217-27 Citation Subset: IM |
Affiliation:
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Department of Neurosciences, School of Medicine, University of California, San Diego, La Jolla 92093-0624. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Alkaloids
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pharmacology* Antibodies, Monoclonal Antigens, CD3 Cell Death / drug effects Cyclosporine / pharmacology* Dose-Response Relationship, Drug Enzyme Activation / drug effects Genistein Hybridomas / immunology Isoflavones / pharmacology Lymphocyte Activation / immunology* Phorbol Esters / pharmacology Protein Kinase C / immunology* Staurosporine T-Lymphocytes / immunology* |
| Grant Support | |
ID/Acronym/Agency:
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AG08205/AG/NIA NIH HHS; NS28121/NS/NINDS NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Alkaloids; 0/Antibodies, Monoclonal; 0/Antigens, CD3; 0/Isoflavones; 0/Phorbol Esters; 446-72-0/Genistein; 59865-13-3/Cyclosporine; 62996-74-1/Staurosporine; EC 2.7.11.13/Protein Kinase C |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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