Document Detail

The involvement of protein kinase C in activation-induced cell death in T-cell hybridoma.
MedLine Citation:
PMID:  1394438     Owner:  NLM     Status:  MEDLINE    
T-cell hybridoma activated by a variety of stimuli such as anti-cell surface antigen, notably CD3 and T-cell receptors, and Con A undergoes a cell lysis process called activation-induced cell death (AICD). It was found that the major protein kinase C (PKC) isoform in the 2B4.11 T-cell hybridoma, PKC(alpha), was translocated from the cytosolic to the particulate fraction when these hybridoma cells were induced to die by plastic-adsorbed anti-CD3 antibodies. Inhibitors of protein phosphorylation rescued 2B4.11 cells from AICD as determined by the analysis of cellular metabolism and the proportion of living cells. Furthermore, PKC(alpha) down-regulation by phorbol ester treatment abolished AICD, and the degree of PKC down-regulation correlated well with the degree of AICD abolishment, suggesting that PKC activation represents an essential step in the molecular mechanisms underlying AICD in this T-cell hybridoma.
L W Jin; K Inaba; T Saitoh
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Cellular immunology     Volume:  144     ISSN:  0008-8749     ISO Abbreviation:  Cell. Immunol.     Publication Date:  1992 Oct 
Date Detail:
Created Date:  1992-10-28     Completed Date:  1992-10-28     Revised Date:  2007-11-15    
Medline Journal Info:
Nlm Unique ID:  1246405     Medline TA:  Cell Immunol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  217-27     Citation Subset:  IM    
Department of Neurosciences, School of Medicine, University of California, San Diego, La Jolla 92093-0624.
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MeSH Terms
Alkaloids / pharmacology*
Antibodies, Monoclonal
Antigens, CD3
Cell Death / drug effects
Cyclosporine / pharmacology*
Dose-Response Relationship, Drug
Enzyme Activation / drug effects
Hybridomas / immunology
Isoflavones / pharmacology
Lymphocyte Activation / immunology*
Phorbol Esters / pharmacology
Protein Kinase C / immunology*
T-Lymphocytes / immunology*
Grant Support
Reg. No./Substance:
0/Alkaloids; 0/Antibodies, Monoclonal; 0/Antigens, CD3; 0/Isoflavones; 0/Phorbol Esters; 446-72-0/Genistein; 59865-13-3/Cyclosporine; 62996-74-1/Staurosporine; EC Kinase C

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