| An investigation into the preservation of microbial cell banks for α-amylase production during 5 l fed-batch Bacillus licheniformis fermentations. | |
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MedLine Citation:
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PMID: 20490614 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Fluorescent staining techniques were used for a systematic examination of methods used to cryopreserve microbial cell banks. The aim of cryopreservation here is to ensure subsequent reproducible fermentation performance rather than just post thaw viability. Bacillus licheniformis cell physiology post-thaw is dependent on the cryopreservant (either Tween 80, glycerol or dimethyl sulphoxide) and whilst this had a profound effect on the length of the lag phase, during subsequent 5 l fed-batch fermentations, it had little effect on maximum specific growth rate, final biomass concentration or α-amylase activity. Tween 80 not only protected the cells during freezing but also helped them recover post-thaw resulting in shorter process times. |
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Authors:
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Nichola H Hancocks; Colin R Thomas; Stuart M Stocks; Christopher J Hewitt |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2010-05-20 |
Journal Detail:
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Title: Biotechnology letters Volume: 32 ISSN: 1573-6776 ISO Abbreviation: Biotechnol. Lett. Publication Date: 2010 Oct |
Date Detail:
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Created Date: 2010-09-17 Completed Date: 2011-01-10 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 8008051 Medline TA: Biotechnol Lett Country: Netherlands |
Other Details:
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Languages: eng Pagination: 1405-12 Citation Subset: IM |
Affiliation:
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School of Chemical Engineering, University of Birmingham, Edgbaston, Birmingham, B15 2TT, UK. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Bacillus
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enzymology*,
growth & development,
metabolism Bacterial Proteins / metabolism* Biomass Biotechnology / methods* Cryopreservation / methods* Fermentation Fluorescence Staining and Labeling alpha-Amylases / metabolism* |
| Grant Support | |
ID/Acronym/Agency:
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//Biotechnology and Biological Sciences Research Council |
| Chemical | |
Reg. No./Substance:
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0/Bacterial Proteins; EC 3.2.1.1/alpha-Amylases |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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