Document Detail

An investigation of hormesis of trichloroethylene in L-02 liver cells by differential proteomic analysis.
MedLine Citation:
PMID:  19109764     Owner:  NLM     Status:  MEDLINE    
Hormesis is the dose-response pattern of the biological responses to toxic chemicals, characterized by low-dose stimulation and high-dose inhibition. Although it is known that some cell types exhibit an adaptive response to low levels of cytotoxic agents, its molecular mechanism is still unclear and it has yet to be established whether this is a universal phenomenon that occurs in all cell types in response to exposure to every chemical. Trichloroethylene (TCE) is an organic solvent widely used and is released into the atmosphere from industrial degreasing operations. Acute (short-term) and chronic (long-term) inhalation exposure to trichloroethylene can affect the human health. In order to elucidate a cell-survival adaptive response of L-02 liver cells exposed to low dose of TCE, CCK-8 assay was used to assess cytotoxicity, and examined the possible mechanisms of hormesis by proteomics technology. We found that exposure of L-02 liver cells to low level of TCE resulted in adaptation to further exposure to higher level, about 1,000 protein-spots were obtained by two-dimensional electrophoresis (2-DE) and five protein spots were identified by matrix-assisted laser desorption/ionization mass spectrometry and tandem mass spectrometry sequencing of tryptic peptides. Our results suggest that a relationship may exist between identified proteins and TCE-induced hormesis, which are very useful for further study of the mechanism and risk assessment of TCE.
Hai-Yan Huang; Jian-Jun Liu; Ren-Rong Xi; Xiu-Mei Xing; Jian-Hui Yuan; Lin-Qing Yang; Gong-Hua Tao; Chun-Mei Gong; Zhi-Xiong Zhuang
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2008-12-25
Journal Detail:
Title:  Molecular biology reports     Volume:  36     ISSN:  1573-4978     ISO Abbreviation:  Mol. Biol. Rep.     Publication Date:  2009 Nov 
Date Detail:
Created Date:  2009-10-21     Completed Date:  2010-02-02     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0403234     Medline TA:  Mol Biol Rep     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  2119-29     Citation Subset:  IM    
Toxicology Laboratory, Shenzhen Center for Disease Control and Prevention, 518020 Shenzhen, Guangdong, People's Republic of China.
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MeSH Terms
Analysis of Variance
Base Sequence
Blotting, Western
Cell Line
Dose-Response Relationship, Drug
Electrophoresis, Gel, Two-Dimensional
Glutathione Transferase / genetics,  metabolism
Hepatocytes / drug effects*,  metabolism
Molecular Sequence Data
Proteins / genetics,  metabolism
Proteome / drug effects*,  metabolism
Proteomics / methods*
Reproducibility of Results
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
Trichloroethylene / pharmacology*
Reg. No./Substance:
0/Proteins; 0/Proteome; 79-01-6/Trichloroethylene; EC Transferase

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