Document Detail

An intron in a ribosomal protein gene from Tetrahymena.
MedLine Citation:
PMID:  16453715     Owner:  NLM     Status:  PubMed-not-MEDLINE    
We have cloned and sequenced a single copy gene encoding a ribosomal protein from the ciliate Tetrahymena thermophila. The gene product was identified as ribosomal protein S25 by comparison of the migration in two-dimensional polyacrylamide gels of the protein synthesized by translation in vitro of hybrid-selected mRNA and authentic ribosomal proteins. The proteins show strong homology to ribosomal protein S12 from Escherichia coli. The coding region of the gene is interrupted by a 979-bp intron 68 bp downstream of the translation start. This is the first intron in a protein encoding gene of a ciliate to be described at the nucleotide sequence level. The intron obeys the GT/AG rule for splice junctions of nuclear mRNA introns from higher eukaryotes but lacks the pyrimidine stretch usually found in the immediate vicinity of the 3' splice junction. The structure of the intron and the fact that it is found together with the well described self-splicing rRNA intron is discussed in relation to the evolution of RNA splicing.
H Nielsen; P H Andreasen; H Dreisig; K Kristiansen; J Engberg
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  The EMBO journal     Volume:  5     ISSN:  0261-4189     ISO Abbreviation:  EMBO J.     Publication Date:  1986 Oct 
Date Detail:
Created Date:  2010-06-28     Completed Date:  2010-07-08     Revised Date:  2010-09-20    
Medline Journal Info:
Nlm Unique ID:  8208664     Medline TA:  EMBO J     Country:  England    
Other Details:
Languages:  eng     Pagination:  2711-7     Citation Subset:  -    
Department of Biochemistry B, Panum Institute, University of Copenhagen, Blegdamsvej 3C, DK-2200 Copenhagen N, Denmark.
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