Document Detail

The intragastric host-mediated assay for the assessment of the formation of direct mutagens in vivo.
MedLine Citation:
PMID:  6828041     Owner:  NLM     Status:  MEDLINE    
The intragastric host-mediated assay (h.m.a.) was devised and carried out with a view to assessing the formation of direct mutagens in the gastrointestinal tract of mammals. The h.m.a. consists in the injection of nitrosable compounds, NaNO2 and cells of the yeast S. pombe, by gavage into the animals' stomachs and in the recovery of the target cells from the faeces for mutation-induction analysis. Methylurea was chosen as a model nitrosable compound, and the effects of nitrosation modulators such as ascorbic acid and thiocyanate were studied. Cimetidine, a drug nitrosable in vitro, was tested with the system. Positive results were obtained only at very large doses and in artificially produced low pH. The new host-mediated assay seems to be efficient in revealing the formation, in vivo, of direct, short-living mutagens.
R Barale; D Zucconi; M Romano; N Loprieno
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Mutation research     Volume:  113     ISSN:  0027-5107     ISO Abbreviation:  Mutat. Res.     Publication Date:  1983 Feb 
Date Detail:
Created Date:  1983-04-07     Completed Date:  1983-04-07     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0400763     Medline TA:  Mutat Res     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  21-32     Citation Subset:  IM    
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MeSH Terms
Ascorbic Acid / pharmacology
Cimetidine / adverse effects,  metabolism
Methylurea Compounds / metabolism
Mutagens / metabolism*
Schizosaccharomyces / metabolism
Sodium Nitrite / metabolism
Stomach / metabolism*
Thiocyanates / pharmacology
Reg. No./Substance:
0/Methylurea Compounds; 0/Mutagens; 0/Thiocyanates; 50-81-7/Ascorbic Acid; 51481-61-9/Cimetidine; 7632-00-0/Sodium Nitrite

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