Document Detail

The intracellular pH of human leucocytes in response to acid-base changes in vitro.
MedLine Citation:
PMID:  4259     Owner:  NLM     Status:  MEDLINE    
1. Viable human leucocytes were isolated from venous blood and suspended in artificial media. Intracellular pH measurements were made by the dimethyloxazolidinedione technique in conditions simulating "respiratory" or "metabolic" acid-base disturbances. 2. Normal intracellular pH was 7-11 +/- 0-02 (mean +/- 2 SD) at an extracellular PCO2 of 5-8 kPa and a bicarbonate concentration of 25 mmol/l. 3. "Respiratory" and "metabolic" acidosis caused little change in pHi although increases in PCO2 led to relatively greater falls in pHi than did reduction in external bicarbonate concentration. 4. "Respiratory" and "metabolic" alkalosis caused similar and relatively greater increases in the pHi when compared with the response to an external acidosis.
G E Levin; P Collinson; D N Baron
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Clinical science and molecular medicine     Volume:  50     ISSN:  0301-0538     ISO Abbreviation:  Clin Sci Mol Med     Publication Date:  1976 Apr 
Date Detail:
Created Date:  1976-07-06     Completed Date:  1976-07-06     Revised Date:  2004-11-17    
Medline Journal Info:
Nlm Unique ID:  0367540     Medline TA:  Clin Sci Mol Med     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  293-9     Citation Subset:  IM    
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MeSH Terms
Acid-Base Equilibrium*
Bicarbonates / pharmacology
Carbon Dioxide / blood
Hydrogen-Ion Concentration
Leukocytes / drug effects,  metabolism*
Partial Pressure
Reg. No./Substance:
0/Bicarbonates; 124-38-9/Carbon Dioxide

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