| The intracellular amino acid pools of Giardia intestinalis, Trichomonas vaginalis, and Crithidia luciliae. | |
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MedLine Citation:
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PMID: 8056076 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The total intracellular amino acid profiles of Giardia intestinalis trophozoites, Trichomonas vaginalis, and Crithidia luciliae were determined by sensitive amino acid analysis. The three protozoan parasites exhibited distinctively different amino acid profiles, but all three were dominated by high concentrations of intracellular alanine. This common feature suggests that alanine synthesis is a major aspect of intermediary metabolism in these protozoan parasites. There were also distinctively different aspects, particularly those related to arginine metabolism. Ornithine, citrulline, and ammonia were found in G. intestinalis trophozoites, but no intracellular arginine was detected. This pattern is consistent with the high activity of giardial arginine deiminase and the arginine dihydrolase pathway. However, in contrast, both T. vaginalis and C. luciliae contained considerable intracellular pools of arginine. When the G. intestinalis trophozoites were divided into the two populations existing in in vitro culture--attached and nonattached--there were no significant differences between the amino acid profiles of the two populations, with the exception of citrulline, which was found in lower concentrations in the nonattached cells. The T. vaginalis profile was characterised by high concentrations of valine and leucine, whereas the C. luciliae profile was dominated by high levels of glutamate and proline. Overall, the analysis of the total amino acid pool provides a valuable technique to rapidly highlight those amino acids of potential metabolic significance and to provide a rapid technique for defining the nature of amino acid metabolic interactions in situ. |
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Authors:
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L A Knodler; M R Edwards; P J Schofield |
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Publication Detail:
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Type: Comparative Study; Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Experimental parasitology Volume: 79 ISSN: 0014-4894 ISO Abbreviation: Exp. Parasitol. Publication Date: 1994 Sep |
Date Detail:
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Created Date: 1994-09-13 Completed Date: 1994-09-13 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0370713 Medline TA: Exp Parasitol Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 117-25 Citation Subset: IM |
Affiliation:
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School of Biochemistry and Molecular Genetics, University of New South Wales, Kensington, Australia. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Amino Acids
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analysis* Animals Crithidia / chemistry* Giardia lamblia / chemistry* Intracellular Fluid / chemistry Trichomonas vaginalis / chemistry* |
| Chemical | |
Reg. No./Substance:
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0/Amino Acids |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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