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The internalization pathway, metabolic fate and biological effect of superparamagnetic iron oxide nanoparticles in the macrophage-like RAW264.7 cell.
MedLine Citation:
PMID:  21922429     Owner:  NLM     Status:  In-Data-Review    
Abstract/OtherAbstract:
The potential applications of superparamagnetic iron oxide nanoparticles (SPIONs) in several nanomedical fields have attracted intense interest based on the cell-nano interaction. However, the mechanisms underlying cell uptake, the intracellular trail, final fate and the biological effects of SPIONs have not yet been clearly elucidated. Here, we showed that multiple endocytic pathways were involved in the internalization process of SPIONs in the RAW264.7 macrophage. The internalized SPIONs were biocompatible and used three different metabolic pathways: The SPIONs were distributed to daughter cells during mitosis; they were degraded in the lysosome and free iron was released into the intracellular iron metabolic pool; and, the intact SPIONs were potentially exocytosed out of the cells. The internalized SPIONs did not induce cell damage but affected iron metabolism, inducing the upregulation of ferritin light chain at both the mRNA and protein levels and ferroportin 1 at the mRNA level. These results may contribute to the development of nanobiology and to the safe use of SPIONs in medicine when administered as a contrast medium or a drug delivery tool.
Authors:
Jingli Gu; Haifei Xu; Yehua Han; Wei Dai; Wei Hao; Chunyu Wang; Ning Gu; Haiyan Xu; Jimin Cao
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Publication Detail:
Type:  Journal Article     Date:  2011-09-16
Journal Detail:
Title:  Science China. Life sciences     Volume:  54     ISSN:  1869-1889     ISO Abbreviation:  Sci China Life Sci     Publication Date:  2011 Sep 
Date Detail:
Created Date:  2011-09-16     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101529880     Medline TA:  Sci China Life Sci     Country:  China    
Other Details:
Languages:  eng     Pagination:  793-805     Citation Subset:  IM    
Affiliation:
Department of Physiology and Pathophysiology, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences, School of Basic Medicine Peking Union Medical College, Beijing, 100005, China.
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