| The internalization and metabolism of 3-deoxyglucosone in human umbilical vein endothelial cells. | |
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MedLine Citation:
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PMID: 16452312 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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3-Deoxyglucosone (3-DG), a dicarbonyl compound produced by glycation, plays a role in the modification and cross-linking of long-lived proteins. We synthesized [3H]3-DG from [3H]glucose and developed an internalization assay system using HPLC to examine its cellular metabolism. When smooth muscle cells or human umbilical vein endothelial cells were incubated with [3H]3-DG, it was found that [3H]3-DG was internalized by cells in a time dependent manner. The rate of internalization was reduced when the cells were incubated at 4 degrees C or treated with phenylarsine oxide (PAO). By monitoring [3H]3-DG taken up by cells, it was confirmed that 3-DG is reduced to 3-deoxyfructose (3-DF) and that this reaction was inhibited by an aldo-keto reductase inhibitor (ARI). The presence of 3-DG led to an increase in reactive oxygen species levels in the cells and subsequent apoptosis, and the effect was enhanced by pretreatment with ARI. These results suggest that 3-DG is internalized by cells and reduced to 3-DF by aldo-keto reductases, and that the internalized 3-DG is responsible for the production of intracellular oxidative stress. |
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Authors:
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Haruhiko Sakiyama; Motoko Takahashi; Toshihiro Yamamoto; Tadashi Teshima; Seung Ho Lee; Yasuhide Miyamoto; Yoshiko Misonou; Naoyuki Taniguchi |
Publication Detail:
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Type: Comparative Study; Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Journal of biochemistry Volume: 139 ISSN: 0021-924X ISO Abbreviation: J. Biochem. Publication Date: 2006 Feb |
Date Detail:
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Created Date: 2006-02-02 Completed Date: 2007-01-05 Revised Date: 2007-12-19 |
Medline Journal Info:
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Nlm Unique ID: 0376600 Medline TA: J Biochem Country: Japan |
Other Details:
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Languages: eng Pagination: 245-53 Citation Subset: IM |
Affiliation:
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Department of Biochemistry, Osaka University Graduate School of Medicine, B1, 2-2 Yamadaoka, Suita, Osaka 565-0871, Japan. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Cells, Cultured Deoxyglucose / analogs & derivatives*, chemistry, metabolism, pharmacokinetics Dose-Response Relationship, Drug Endothelial Cells / cytology, drug effects, metabolism* Humans Muscle, Smooth, Vascular / cytology, drug effects, metabolism Time Factors Tritium Umbilical Veins / cytology, drug effects, metabolism* |
| Chemical | |
Reg. No./Substance:
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10028-17-8/Tritium; 154-17-6/Deoxyglucose; 4084-27-9/3-deoxyglucosone |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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