Document Detail


The intermediate filament nestin is highly expressed in normal human podocytes and podocytes in glomerular disease.
MedLine Citation:
PMID:  17929992     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The intermediate protein nestin is expressed in proliferating embryonic tissues and adult tissues undergoing repair. Recently this protein been identified in rodent podocytes. Its role in this cell is unknown, since podocytes are believed to be terminally differentiated and nondividing. We report the first study of nestin in human kidney. Nestin expression in normal mature human glomeruli was confined to podocytes. In developing kidney, nestin was detected in metanephric blastema and in podocytic cells at all stages of glomerular development. Nestin co-localized with vimentin but not with actin or heavy chain myosin IIA, using a mouse podocyte cell line. Knockdown of nestin in a murine podocyte cell line failed to produce any obvious phenotypic change or alteration in vimentin distribution but was associated with increased cell cycling. A survey of glomerular diseases failed to identify any condition lacking nestin, indicating that the protein is critical for some aspect of podocyte function. Perhaps through an association with vimentin, nestin serves to bolster the mechanical strength of these cells that experience high tensile stress during glomerular filtration. Nestin was also expressed in podocytes that are reported to be 'dysregulated' (lacking podocyte markers). Thus, nestin has a potential as a reliable podocyte marker, even for podocytes that are not completely differentiated (for example, during development) or 'dedifferentiated' in glomerular disease.
Authors:
Julie Perry; Michael Ho; Sandra Viero; Keqin Zheng; Robert Jacobs; Paul S Thorner
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Pediatric and developmental pathology : the official journal of the Society for Pediatric Pathology and the Paediatric Pathology Society     Volume:  10     ISSN:  1093-5266     ISO Abbreviation:  Pediatr. Dev. Pathol.     Publication Date:    2007 Sep-Oct
Date Detail:
Created Date:  2007-10-12     Completed Date:  2007-12-12     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9809673     Medline TA:  Pediatr Dev Pathol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  369-82     Citation Subset:  IM    
Affiliation:
Division of Pathology, Hospital for Sick Children, Toronto, ON, Canada.
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MeSH Terms
Descriptor/Qualifier:
Adolescent
Animals
Cell Line
Child
Child, Preschool
Gene Expression
Gene Expression Regulation, Developmental
Humans
Immunohistochemistry
Infant
Intermediate Filament Proteins / metabolism*
Kidney Diseases / metabolism*
Mice
Nerve Tissue Proteins / metabolism*
Podocytes / metabolism*,  ultrastructure
RNA, Small Interfering
Chemical
Reg. No./Substance:
0/Intermediate Filament Proteins; 0/Nerve Tissue Proteins; 0/RNA, Small Interfering; 0/nestin

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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