Document Detail

The interferon-inducible RNA helicase, mda-5, is involved in measles virus-induced expression of antiviral cytokines.
MedLine Citation:
PMID:  16782388     Owner:  NLM     Status:  MEDLINE    
Activation of host cell antiviral responses is mediated by receptors detecting the presence of viruses. Here we have studied the role of double-stranded RNA (dsRNA) binding molecules melanoma differentiation-associated gene 5 (mda-5), retinoic acid inducible gene I (RIG-I), and Toll-like receptor 3 (TLR3) in measles virus (MV)-induced expression of antiviral cytokines and chemokines in human A549 lung epithelial cells and human umbilical vein endothelial cells (HUVECs). We show that MV infection results in the activation of mda-5, RIG-I, and TLR3 gene expression that is followed by high expression of interferon (IFN)-beta, interleukin (IL)-28 and IL-29, CCL5, and CXCL10 genes. We also demonstrate that IFN-alpha and IFN-beta upregulate mda-5, RIG-I, and TLR3 gene expression in epithelial and endothelial cell lines. Forced expression of mda-5, but not that of RIG-I or TLR3, leads to enhanced IFN-beta promoter activity in MV-infected A549 cells. Our results suggest that IFN-inducible mda-5 is involved in MV-induced expression of antiviral cytokines.
Heidi Berghäll; Jukka Sirén; Devanand Sarkar; Ilkka Julkunen; Paul B Fisher; Raija Vainionpää; Sampsa Matikainen
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2006-05-26
Journal Detail:
Title:  Microbes and infection / Institut Pasteur     Volume:  8     ISSN:  1286-4579     ISO Abbreviation:  Microbes Infect.     Publication Date:  2006 Jul 
Date Detail:
Created Date:  2006-09-11     Completed Date:  2007-01-16     Revised Date:  2009-11-19    
Medline Journal Info:
Nlm Unique ID:  100883508     Medline TA:  Microbes Infect     Country:  France    
Other Details:
Languages:  eng     Pagination:  2138-44     Citation Subset:  IM    
Department of Virology, University of Turku, Kiinamyllynkatu 13, FIN-20520 Turku, Finland.
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MeSH Terms
Blotting, Northern
Blotting, Western
Cell Line
Chemokines / biosynthesis
Cytokines / biosynthesis*
DEAD-box RNA Helicases / immunology*
Endothelial Cells / immunology
Epithelial Cells / immunology
Gene Expression
Measles virus / immunology*
RNA, Messenger / analysis,  genetics
Toll-Like Receptor 3 / immunology
Reg. No./Substance:
0/Chemokines; 0/Cytokines; 0/RNA, Messenger; 0/Toll-Like Receptor 3; EC 3.6.1.-/DEAD-box RNA Helicases; EC 3.6.1.-/IFIH1 protein, human

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