| The interferon-inducible RNA helicase, mda-5, is involved in measles virus-induced expression of antiviral cytokines. | |
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MedLine Citation:
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PMID: 16782388 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Activation of host cell antiviral responses is mediated by receptors detecting the presence of viruses. Here we have studied the role of double-stranded RNA (dsRNA) binding molecules melanoma differentiation-associated gene 5 (mda-5), retinoic acid inducible gene I (RIG-I), and Toll-like receptor 3 (TLR3) in measles virus (MV)-induced expression of antiviral cytokines and chemokines in human A549 lung epithelial cells and human umbilical vein endothelial cells (HUVECs). We show that MV infection results in the activation of mda-5, RIG-I, and TLR3 gene expression that is followed by high expression of interferon (IFN)-beta, interleukin (IL)-28 and IL-29, CCL5, and CXCL10 genes. We also demonstrate that IFN-alpha and IFN-beta upregulate mda-5, RIG-I, and TLR3 gene expression in epithelial and endothelial cell lines. Forced expression of mda-5, but not that of RIG-I or TLR3, leads to enhanced IFN-beta promoter activity in MV-infected A549 cells. Our results suggest that IFN-inducible mda-5 is involved in MV-induced expression of antiviral cytokines. |
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Authors:
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Heidi Berghäll; Jukka Sirén; Devanand Sarkar; Ilkka Julkunen; Paul B Fisher; Raija Vainionpää; Sampsa Matikainen |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2006-05-26 |
Journal Detail:
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Title: Microbes and infection / Institut Pasteur Volume: 8 ISSN: 1286-4579 ISO Abbreviation: Microbes Infect. Publication Date: 2006 Jul |
Date Detail:
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Created Date: 2006-09-11 Completed Date: 2007-01-16 Revised Date: 2009-11-19 |
Medline Journal Info:
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Nlm Unique ID: 100883508 Medline TA: Microbes Infect Country: France |
Other Details:
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Languages: eng Pagination: 2138-44 Citation Subset: IM |
Affiliation:
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Department of Virology, University of Turku, Kiinamyllynkatu 13, FIN-20520 Turku, Finland. heidi.berghall@utu.fi |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Blotting, Northern Blotting, Western Cell Line Chemokines / biosynthesis Cytokines / biosynthesis* DEAD-box RNA Helicases / immunology* Endothelial Cells / immunology Epithelial Cells / immunology Gene Expression Humans Measles virus / immunology* RNA, Messenger / analysis, genetics Toll-Like Receptor 3 / immunology |
| Chemical | |
Reg. No./Substance:
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0/Chemokines; 0/Cytokines; 0/RNA, Messenger; 0/Toll-Like Receptor 3; EC 3.6.1.-/DEAD-box RNA Helicases; EC 3.6.1.-/IFIH1 protein, human |
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