| The interferon-alpha and interleukin-10 responses in neonates differ from adults, and their production remains partial throughout the first 18 months of life. | |
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MedLine Citation:
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PMID: 20964643 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Previous studies have suggested that the susceptibility of newborns to infections is linked to the immaturity of their immune system, but very few data are available on the early stages of maturation of the immune response. Therefore, we decided to investigate the evolution of the interferon (IFN)-α and interleukin (IL)-10 responses in neonatal mononuclear cells. To this end, mononuclear cells isolated from cord blood and peripheral blood of 2-, 6- and 18-month-old children and adults were stimulated with unmethylated cytosine-phosphate-guanosine oligodeoxynucleotide (CpG-ODN) 2216 (IFN-α response) or lipopolysaccharide (LPS) (IL-10 response) for 24 h. The production of IFN-α and IL-10 was then measured in culture supernatants using enzyme-linked immunosorbent assay (ELISA) or a 6-plex cytokine array, respectively. Compared to adults, we found a significant impairment in both the IFN-α and IL-10 responses of neonatal mononuclear cells. Interestingly, both responses had increased significantly after 2 months, but remained lower than the adult responses throughout the first 18 months of life. This study shows that although the immune response of neonates tends to mature fairly quickly, it remains different when compared to the adult immune response throughout the first 18 months of life. This could have important consequences on children's ability to mount an appropriate immune response to various challenges and to establish tolerance and immune homeostasis. |
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Authors:
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O Vosters; C Lombard; F André; G Sana; E M Sokal; F Smets |
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Publication Detail:
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Type: Comparative Study; Journal Article; Research Support, Non-U.S. Gov't Date: 2010-10-21 |
Journal Detail:
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Title: Clinical and experimental immunology Volume: 162 ISSN: 1365-2249 ISO Abbreviation: Clin. Exp. Immunol. Publication Date: 2010 Dec |
Date Detail:
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Created Date: 2010-11-12 Completed Date: 2011-03-08 Revised Date: 2011-12-21 |
Medline Journal Info:
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Nlm Unique ID: 0057202 Medline TA: Clin Exp Immunol Country: England |
Other Details:
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Languages: eng Pagination: 494-9 Citation Subset: IM |
Copyright Information:
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© 2010 The Authors. Clinical and Experimental Immunology © 2010 British Society for Immunology. |
Affiliation:
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Université catholique de Louvain and Cliniques Universitaires Saint-Luc, Laboratory of Pediatric Hepatology and Cell Therapy, PEDI Unit, Brussels, Belgium. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Adult Cells, Cultured Disease Susceptibility Fetal Blood / cytology Humans Infant Infection / immunology* Interferon-alpha / biosynthesis*, genetics, secretion Interleukin-10 / biosynthesis*, genetics, secretion Leukocytes, Mononuclear / drug effects, immunology, metabolism*, pathology Lipopolysaccharides / pharmacology Lymphocyte Activation / drug effects Oligodeoxyribonucleotides / pharmacology Time Factors |
| Chemical | |
Reg. No./Substance:
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0/CpG ODN 2216; 0/Interferon-alpha; 0/Lipopolysaccharides; 0/Oligodeoxyribonucleotides; 130068-27-8/Interleukin-10 |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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