| The interference of heparin on IEF-PAGE of erythropoietins. | |
| | |
MedLine Citation:
|
PMID: 21204288 Owner: NLM Status: In-Process |
Abstract/OtherAbstract:
|
Because of the risk of suffering a stroke or heart attack, some athletes and their medical supervisors admitted having used anticoagulants (e.g. acetylsalicylic acid) in combination with doping with recombinant erythropoietins (rhEPO). Heparin is one of the oldest and cheapest anticoagulants. The anticoagulative effect of heparin is a result of the binding of heparin to the plasma protein antithrombin III and the subsequent inactivation of blood clotting factors (e.g. factor IIa, IXa, Xa, XIa, XIIa). Heparin-a polyanion-is known to interact with carrier ampholytes used in IEF-PAGE. Two different types of heparin pharmaceuticals are used for medical purposes: unfractionated heparins (UFH) and low molecular weight heparins (LMWH). Their influence on IEF- and SDS-PAGE was investigated. Only UFH had a profound impact on IEF-PAGE, leading to excessive smearing or complete abolishment of the EPO IEF-profile and shifting of acidic EPO-isoforms in the endogenous region of the gel. No such effect was observable for SDS-PAGE. Remedies include immunoaffinity purification of EPO before IEF-PAGE or the treatment of the urinary retentate with solid urea. A combined usage of IEF- and SDS-PAGE is recommended for confirming the presence of rhEPO in urine and for further analysis of smearing (and therefore suspicious) samples. This two-method approach is already in accordance with the technical document on EPO-analysis (TD2009EPO) of the World Anti-Doping Agency (WADA). |
| | |
Authors:
|
Christian Reichel; Edmund Benetka; Thomas Geisendorfer; Veronika Scheiblhofer; Friedrich Abzieher |
Related Documents
:
|
11852748 - Do persons with diabetes know their (a1c) number? 12605208 - Cardioactive steroid poisoning from an herbal cleansing preparation. 75128 - Comparative ultrastructural morphometric studies of micromyeloblastoid and lymphoblasto... 16540438 - Effect of stiffness and movement speed on selected dynamic torque characteristics of hy... 19546128 - The transformation of vascular surgeons to vascular specialists: policy or necessity? 11089698 - The value of good medical student teaching: increasing the number of radiology residenc... |
Publication Detail:
|
Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2010-11-11 |
Journal Detail:
|
Title: Drug testing and analysis Volume: 2 ISSN: 1942-7611 ISO Abbreviation: Drug Test Anal Publication Date: 2010 Nov-Dec |
Date Detail:
|
Created Date: 2011-01-04 Completed Date: - Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 101483449 Medline TA: Drug Test Anal Country: England |
Other Details:
|
Languages: eng Pagination: 568-75 Citation Subset: IM |
Copyright Information:
|
Copyright © 2010 John Wiley & Sons, Ltd. |
Affiliation:
|
Doping Control Laboratory, AIT Seibersdorf Laboratories, Seibersdorf, Austria. christian.reichel@seibersdorf-laboratories.at |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
|
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Implementation of the biological passport: the experience of the International Cycling Union.
Next Document: Characterization of microbial contaminants in urine.