Document Detail


The interference of gingival cell cultures with growth of selected bacteria.
MedLine Citation:
PMID:  8703443     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The aim of the present study was to analyze the interference of oral tissue cells or cell lines (effector cells) with growth of reference bacteria, and furthermore to investigate whether cells derived from different individuals differ in such activity. The reference bacteria were Staphylococcus aureus, Staphylococcus epidermidis, Streptococcus mitis, Actinobacillus actinomycetemcomitans, Porphyromonas gingivalis, and Fusobacterium nucleatum. The effector cells used were gingival fibroblasts (GF) from 21 periodontally involved persons, gingival epithelial cells (E) from 2 such persons, HeLa cells (HeLa), and an amnion cell line (Amnion). The cells were cultivated and their supernatants tested for antibacterial activity in a Bioscreen robot analyzer (Labsystems, Finland). Results suggest that the antibacterial activity of each tested primary cell line of tissue had its own profile depending on cell type and donor, and that the composition of oral microbiota was influenced by oral cells, which might, in turn, contribute to the variations in the pathogenesis of periodontal diseases.
Authors:
A Johansson; A Bergenholtz; S E Holm
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  APMIS : acta pathologica, microbiologica, et immunologica Scandinavica     Volume:  104     ISSN:  0903-4641     ISO Abbreviation:  APMIS     Publication Date:  1996 May 
Date Detail:
Created Date:  1996-09-09     Completed Date:  1996-09-09     Revised Date:  2004-11-17    
Medline Journal Info:
Nlm Unique ID:  8803400     Medline TA:  APMIS     Country:  DENMARK    
Other Details:
Languages:  eng     Pagination:  367-73     Citation Subset:  IM    
Affiliation:
Department of Periodontology, Umeå University, Sweden.
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MeSH Terms
Descriptor/Qualifier:
Actinobacillus actinomycetemcomitans / growth & development
Bacteria / growth & development*
Cell Culture Techniques
Cell Division
Colony Count, Microbial / instrumentation,  methods
Culture Media / pharmacology
Fusobacterium nucleatum / growth & development
Gingiva / cytology,  microbiology*
Hela Cells
Humans
Porphyromonas gingivalis / growth & development
Staphylococcus aureus / growth & development
Staphylococcus epidermidis / growth & development
Streptococcus / growth & development
Chemical
Reg. No./Substance:
0/Culture Media

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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