Document Detail


The interference of cold ischemia time in the quality of total RNA from frozen tumor samples.
MedLine Citation:
PMID:  22562477     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
Tumor Banks were created to organize the collection, storage and distribution of biological samples from oncological patients, facilitating its use in cancer research. To ensure the quality of the samples from our bank, we implemented standard operating procedures international. In order to evaluate the influence of cold ischemia time (time between surgical removal of the specimen and the snap freezing of the sample) on the quality of the samples (evaluated by measurement integrity of their RNA), collected during 10 months two tumor samples from each donor, one with up to 30 min of cold ischemia and other with exact 45 min, totaling 100 different donors and 200 samples, 40 from each of the following organs: breast, thyroid, stomach, lung and colorectum. We extracted total RNA from the samples and with the aid of a Bioanalyser, evaluate their quality, comparing it with cold ischemia times in different organs. Among the samples up to 30 min and the samples with exact 45 min, we respectively found 63 (64.3 %) and 36 (36 %) with intact RNA, 11 (11.2 %) and 17 (17 %) partially degraded and 24 (24.5 %) and 47 (47 %) degraded (p < 0.001). Thyroid and colorectal samples were more sensitive to variations in cold ischemia time (p = 0.006 and p = 0.03, respectively). Stomach and lungs were less sensitive (p = 0.919 and p = 0.384, respectively). We concluded that the cold ischemia time up to 30 min was more efficient to maintain the integrity of RNA in most samples, and that RNA degradation varied according to the different topographies.
Authors:
Cristiano Ribeiro Viana; Cristovam Scapulatempo Neto; Ligia Maria Kerr; Edenir Inêz Palmero; Marcia Maria Chiquitelli Marques; Tamara Colaiacovo; Abel Feliciano de Queiroz Junior; André Lopes Carvalho; Sheila Aparecida Coelho Siqueira
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-5-5
Journal Detail:
Title:  Cell and tissue banking     Volume:  -     ISSN:  1573-6814     ISO Abbreviation:  -     Publication Date:  2012 May 
Date Detail:
Created Date:  2012-5-7     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  100965121     Medline TA:  Cell Tissue Bank     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Affiliation:
Department of Pathology, Barretos Cancer Hospital (Pio XII Foundation), Barretos, Brazil, crisrviana@yahoo.com.br.
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