Document Detail


The interactions between iophenoxic acid, iopanoic acid, bilirubin and human serum albumin as studied by fluorescence and Sephadex gel filtration.
MedLine Citation:
PMID:  963887     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Iophenoxic acid increases the fluorescence of bilirubin bound to human serum albumin at drug/albumin molar ratios lower than 1, while iopanoic acid decreases it. The fluorescence enhancement results probably from a change in the fluorescence efficiency due to an iophenoxic acid-induced conformational change in the albumin, which in turn causes displacement of bilirubin from the protein. Iophenoxic acid does not affect the high-affinity bilirubin binding site of albumin. Therefore any enhancement in bilirubin fluorescence caused by the drug indicates that bilirubin is bound to the low-affinity binding sites of albumin. The use of iophenoxic acid in the determination of the extent of saturation of the high-affinity bilirubin binding site of albumin may be of value in the clinical management of infants with neonatal jaundice.
Authors:
D J Birkett; J Kapitulnik
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Clinica chimica acta; international journal of clinical chemistry     Volume:  71     ISSN:  0009-8981     ISO Abbreviation:  Clin. Chim. Acta     Publication Date:  1976 Sep 
Date Detail:
Created Date:  1976-12-01     Completed Date:  1976-12-01     Revised Date:  2004-11-17    
Medline Journal Info:
Nlm Unique ID:  1302422     Medline TA:  Clin Chim Acta     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  129-35     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Bilirubin*
Chromatography, Gel
Cinnamates
Humans
Iodobenzenes*
Iopanoic Acid* / analogs & derivatives*
Protein Binding
Serum Albumin*
Spectrometry, Fluorescence
Chemical
Reg. No./Substance:
0/Cinnamates; 0/Iodobenzenes; 0/Serum Albumin; 635-65-4/Bilirubin; 96-83-3/Iopanoic Acid

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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