| The interaction of triethyl lead with tubulin and microtubules. | |
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MedLine Citation:
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PMID: 3173379 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The impact of triethyl lead chloride was studied on: (i) the in vitro assembly and disassembly of microtubules from porcine brain by turbidometry and electron microscopy, (ii) the microtubule system of living mammalian cells using immunofluorescence microscopy, (iii) cell motility and chemotaxis employing the methods of phagokinetic track formation and the Boyden chamber assay, respectively, and (iv) thiol groups of the protein tubulin by their titration in the presence and absence of the organic lead compound. Triethyl lead chloride inhibited microtubule assembly and depolymerized preformed microtubules in vitro and in living cells. Random motility of cells was not markedly inhibited by triethyl lead chloride, whereas chemotaxis (directed cellular movement) was strongly inhibited. Triethyl lead chloride was found to interact with 2 thiol groups of the tubulin dimer. The interaction of triethyl lead chloride with the tubulin/microtubule system in vivo likely causes aneuploidy and is at least partly responsible for the cytotoxicity of the drug. |
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Authors:
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H P Zimmermann; H Faulstich; G M Hänsch; K H Doenges; C Stournaras |
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Publication Detail:
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Type: In Vitro; Journal Article |
Journal Detail:
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Title: Mutation research Volume: 201 ISSN: 0027-5107 ISO Abbreviation: Mutat. Res. Publication Date: 1988 Oct |
Date Detail:
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Created Date: 1988-11-01 Completed Date: 1988-11-01 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0400763 Medline TA: Mutat Res Country: NETHERLANDS |
Other Details:
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Languages: eng Pagination: 293-302 Citation Subset: IM |
Affiliation:
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Institute of Cell and Tumor Biology, German Cancer Research Center, Heidelberg. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Brain / metabolism Cell Movement / drug effects Cells, Cultured Chemotaxis / drug effects Lead / pharmacology* Microtubules / drug effects* Organometallic Compounds / pharmacology* Swine Tubulin / biosynthesis* |
| Chemical | |
Reg. No./Substance:
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0/Organometallic Compounds; 0/Tubulin; 5224-23-7/triethyllead; 7439-92-1/Lead |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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