| The interaction of human plasma low density lipoproteins with glycosamino-glycans: influence of the chemical composition. | |
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MedLine Citation:
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PMID: 3968986 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Human plasma of 5 normolipemic individuals was incubated for 24 hr at 37 C in the presence or in the absence of lecithin:cholesterol acyltransferase (LCAT)-inhibitors. Plasma stored at 4 C served as a control. The low density lipoprotein (LDL) fractions of the samples were isolated and investigated with respect to changes in chemical composition and complexing activity with glycosamino glycans (GAG). Incubation of plasma in the presence of LCAT inhibitors caused a significant increase of LDL triglycerides at the expense of cholesteryl esters. Incubation with active LCAT not only changed the core but also the surface constituents (decrease in phospholipids and in free cholesterol). The amount of GAG bound per mg of LDL was not uniformly changed in samples incubated after LCAT inhibition. LDL isolated from plasma incubated in the presence of LCAT, on the other hand, showed a significant reduction in GAG binding. The ratio of free cholesterol:GAG in the complex was most significantly reduced in LCAT-modified LDL. There was in addition a highly significant correlation between the LDL:GAG ratio in the complex and the free cholesterol and phospholipid content of the LDL samples. It is concluded that alterations in surface lipid constituents of LDL strongly affect their interaction with sulfated polysaccharides, an effect which may be relevant also in vivo for the interaction of LDL with cell surfaces and intercellular matrices. |
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Authors:
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G M Kostner; R Zechner; M Bihari-Varga; E Gruber |
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Publication Detail:
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Type: In Vitro; Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Lipids Volume: 20 ISSN: 0024-4201 ISO Abbreviation: Lipids Publication Date: 1985 Jan |
Date Detail:
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Created Date: 1985-03-21 Completed Date: 1985-03-21 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0060450 Medline TA: Lipids Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 24-8 Citation Subset: IM |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Female Glycosaminoglycans / metabolism* Humans Lipoproteins, LDL / blood*, metabolism Male Phosphatidylcholine-Sterol O-Acyltransferase / antagonists & inhibitors, metabolism |
| Chemical | |
Reg. No./Substance:
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0/Glycosaminoglycans; 0/Lipoproteins, LDL; EC 2.3.1.43/Phosphatidylcholine-Sterol O-Acyltransferase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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