Document Detail


CD27/CD70 interaction directly drives B cell IgG and IgM synthesis.
MedLine Citation:
PMID:  7589078     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
CD27 is a T cell activation antigen expressed on a majority of peripheral blood T cells. CD27 is also expressed on a subpopulation of human B cells, and it is reported that CD27+ B cells secrete both IgG and IgM. CD70, a ligand for CD27, is expressed on activated T and B cells, suggesting an interaction between T and B cells via CD27/CD70 ligation. Here, we analyze B cell immunoglobulin synthesis using a CD70 transfectant and present functional data showing that B cells secrete large amounts of IgG and IgM as a result of the CD27/CD70 interaction. A flow cytometric analysis showed that CD27 expression was increased and CD70 was expressed on tonsillar and peripheral blood B cells after activation with Staphylococcus aureus Cowan strain (SAC) plus interleukin (IL-2). In addition, the proliferation of B cells was enhanced mildly by the addition of CD70 transfectant, and its proliferation was blocked by anti-CD70 mAb. More importantly, the CD70 transfectant enhanced IgG and IgM production by purified B cells greatly in the presence of SAC plus IL-2. The enhancement was completely blocked by the addition of either anti-CD70 mAb or anti-CD27 mAb. Strongly suggesting that the interaction of CD27 with its ligand, CD70, on B cells plays an important role in B cell growth and differentiation to produce IgG and IgM.
Authors:
K Agematsu; T Kobata; F C Yang; T Nakazawa; K Fukushima; M Kitahara; T Mori; K Sugita; C Morimoto; A Komiyama
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  European journal of immunology     Volume:  25     ISSN:  0014-2980     ISO Abbreviation:  Eur. J. Immunol.     Publication Date:  1995 Oct 
Date Detail:
Created Date:  1995-12-12     Completed Date:  1995-12-12     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  1273201     Medline TA:  Eur J Immunol     Country:  GERMANY    
Other Details:
Languages:  eng     Pagination:  2825-9     Citation Subset:  IM    
Affiliation:
Department of Pediatrics, Shinshu University School of Medicine, Matsumoto, Japan.
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MeSH Terms
Descriptor/Qualifier:
Adult
Antibodies, Monoclonal / pharmacology
Antigens, CD*
Antigens, CD27 / immunology*
Antigens, CD70
B-Lymphocytes / drug effects,  immunology*
Blood Cells
Flow Cytometry
Humans
Immunoglobulin G / biosynthesis*
Immunoglobulin M / biosynthesis*
Interleukin-2 / pharmacology
Ligands
Lymphocyte Activation* / drug effects
Lymphocyte Cooperation
Membrane Proteins / genetics,  immunology*
Palatine Tonsil / cytology
Recombinant Proteins / metabolism
Staphylococcus aureus / immunology
Transfection
Chemical
Reg. No./Substance:
0/Antibodies, Monoclonal; 0/Antigens, CD; 0/Antigens, CD27; 0/Antigens, CD70; 0/CD70 protein, human; 0/Immunoglobulin G; 0/Immunoglobulin M; 0/Interleukin-2; 0/Ligands; 0/Membrane Proteins; 0/Recombinant Proteins

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