Document Detail

The interaction of detergents with bilayer lipid membranes.
MedLine Citation:
PMID:  687619     Owner:  NLM     Status:  MEDLINE    
Detergents are widely used for extracting and purifying membrane proteins. Four such detergents have been studied to find the extent to which they alone can alter black lipid film conductances. The slope of the plot of conductivity versus concentration for Triton X-100 is 4.54 in the range 0.025--0.15 mM; dodecyl sulphate 0.82 in the range 0.01--1 mM; sodium deoxycholate 1.03 in the range 0.01--1 mM and sodium cholate 1.37 in the range 0.1--10 mM. These ranges are below the respective critical micelle concentrations; above these concentrations the membranes break. Bilayer lipid membrane conductivity measured at constant detergent concentration increases with the conductivity of the bathing salt solution with a slope greater than 1, indicating an effect on the putative pore structures induced by detergents.
J A Bangham; E J Lea
Related Documents :
24371039 - Grape-seed procyanidins modulate cellular membrane potential and nutrient-induced glp-1...
17912609 - Comparison of the fluorescence kinetics of detergent-solubilized and membrane-reconstit...
17371059 - Temperature-induced vesicle aggregation in catanionic surfactant systems: the effects o...
12450809 - Temperature- and surfactant-induced membrane modifications that alter listeria monocyto...
21921319 - Poly(ε-caprolactone)/graphene oxide biocomposites: mechanical properties and bioactivity.
8960639 - Quantifying vesicle/mixed micelle partitioning of phosphatidylcholine in model bile by ...
14747359 - Temperature dependency of molecular mobility in preserved seeds.
6366239 - An enzyme-probe method to detect structural changes in the myosin rod.
12828989 - Intracellular localisation of snare proteins in rat parotid acinar cells: snare complex...
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Biochimica et biophysica acta     Volume:  511     ISSN:  0006-3002     ISO Abbreviation:  Biochim. Biophys. Acta     Publication Date:  1978 Aug 
Date Detail:
Created Date:  1978-11-18     Completed Date:  1978-11-18     Revised Date:  2006-04-17    
Medline Journal Info:
Nlm Unique ID:  0217513     Medline TA:  Biochim Biophys Acta     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  388-96     Citation Subset:  IM    
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Cholic Acids
Deoxycholic Acid
Electric Conductivity
Membranes, Artificial*
Polyethylene Glycols
Potassium Chloride
Sodium Chloride
Sodium Dodecyl Sulfate
Reg. No./Substance:
0/Cholic Acids; 0/Detergents; 0/Membranes, Artificial; 0/Phospholipids; 0/Polyethylene Glycols; 151-21-3/Sodium Dodecyl Sulfate; 7447-40-7/Potassium Chloride; 7647-14-5/Sodium Chloride; 83-44-3/Deoxycholic Acid

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Surface potential of phosphatidylserine monolayers. I. Divalent ion binding effect.
Next Document:  Effect of amphotericin B on cholesterol-containing liposomes of egg phosphatidylcholine and didocose...