| The interaction of coagulation factor XII and monocyte/macrophages mediating peritoneal metastasis of epithelial ovarian cancer. | |
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MedLine Citation:
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PMID: 20233624 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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OBJECTIVES: Pathological studies have indicated that the peritoneum of epithelial ovarian cancer (EOC) patients exhibits characteristics of chronic inflammation like peritonitis. Abundant macrophage infiltration and increased expression of coagulation factor XII (FXII) have been observed in the peritoneum of EOC patients. The aim of this study is to determine how the interaction between FXII and monocyte/macrophages (MO/MAs) contributes to EOC cell invasion and metastasis of the peritoneum. METHODS: MO/MAs from the peripheral blood of healthy female donors and tumor-associated macrophages (TAMs) from EOC ascites were collected and cultured. We assessed phenotypes, cytokine/chemokine production, and phagocytic function of FXII-treated MO/MAs. The effects of the FXII-MO/MAs interaction on EOC cell invasion were determined by the Matrigel in vitro invasion assay. In addition, signaling pathway mediators were evaluated for their potential roles in MO/MA activation. RESULTS: MO/MAs exhibited M2-polarized phenotypes after FXII treatment, which was CD163(high)IL-10(high)CCL18(high)IL-8(high)CCR2(high)CXCR2(high). The phagocytic potential of MO/MAs was also upregulated. Matrigel results indicated that invasion of EOC cells was enhanced when exposed to conditioned medium from FXII-stimulated MO/MAs. Transcription factors found to be upregulated in FXII-stimulated MO/MAs included Fra-1, Fra-2, Fos-B in the AP-1 family, oncogenes HIF-1 and Oct, and STAT-5A in the STAT family. CONCLUSIONS: FXII may facilitate EOC cell metastasis by transforming MO/MAs toward tumor-associated macrophage-like cells. |
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Authors:
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Ruili Wang; Ting Zhang; Zhengwen Ma; Ying Wang; Zhongping Cheng; Hong Xu; Weiping Li; Xipeng Wang |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2010-03-16 |
Journal Detail:
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Title: Gynecologic oncology Volume: 117 ISSN: 1095-6859 ISO Abbreviation: Gynecol. Oncol. Publication Date: 2010 Jun |
Date Detail:
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Created Date: 2010-05-14 Completed Date: 2010-05-19 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0365304 Medline TA: Gynecol Oncol Country: United States |
Other Details:
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Languages: eng Pagination: 460-6 Citation Subset: IM |
Copyright Information:
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Crown Copyright 2010. Published by Elsevier Inc. All rights reserved. |
Affiliation:
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Department of Obstetrics and Gynecology, Renji Hospital, Shanghai JiaoTong University School of Medicine, Shanghai, 200001, China. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Antigens, CD
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biosynthesis Cell Line, Tumor Cells, Cultured Cytokines / biosynthesis Factor XII / immunology*, pharmacology Female Humans Macrophages / drug effects, immunology* Neoplasm Invasiveness Neoplasm Metastasis Ovarian Neoplasms / blood*, immunology, pathology* Peritoneal Neoplasms / blood*, immunology, secondary* Phagocytosis Transcription Factors / biosynthesis |
| Chemical | |
Reg. No./Substance:
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0/Antigens, CD; 0/Cytokines; 0/Transcription Factors; 9001-30-3/Factor XII |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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