Document Detail

Lipopolythiourea/DNA interaction: a biophysical study.
MedLine Citation:
PMID:  20227164     Owner:  NLM     Status:  MEDLINE    
Lipopolythioureas (LPT) are original non cationic systems representing an alternative to cationic lipids. Their high transfection efficiency prompted us to investigate further their biophysical properties, and in particular how thiourea lipids interact with DNA. The interaction of lipopolythiourea with DNA was investigated by fluorescence correlation microscopy (FCS). Influence of the lipid length and nature of the thiourea head on the thiourea/DNA interaction were studied. FCS revealed a strong interaction between lipopolythiourea and DNA, occurring at 1 equivalent of a thiourea lipid by a DNA phosphate group, and leading to a condensed plasmid state. From previous in vitro experiments, we could conclude that the lipid leading to the more condensed state of DNA was also the more efficient to transfect cells.
Teresa Kral; Jeanne Leblond; Martin Hof; Daniel Scherman; Jean Herscovici; Nathalie Mignet
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2010-02-25
Journal Detail:
Title:  Biophysical chemistry     Volume:  148     ISSN:  1873-4200     ISO Abbreviation:  Biophys. Chem.     Publication Date:  2010 May 
Date Detail:
Created Date:  2010-04-19     Completed Date:  2010-07-01     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0403171     Medline TA:  Biophys Chem     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  68-73     Citation Subset:  IM    
J. Heyrovsk? Institute of Physical Chemistry, Academy of Sciences of the Czech Republic, Dolejskova 3, Prague 8, Czech Republic.
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MeSH Terms
Biophysical Processes*
DNA / metabolism*
Electrophoresis, Agar Gel
Lipids / chemistry*
Phosphates / metabolism
Spectrometry, Fluorescence
Thiourea / chemistry*,  metabolism*
Reg. No./Substance:
0/Lipids; 0/Phosphates; 62-56-6/Thiourea; 9007-49-2/DNA

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