| The interaction of N,N'-dicyclohexylcarbodiimide with chloroplast coupling factor 1. | |
MedLine Citation:
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PMID: 6444296 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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1. Incubation of soluble spinach Coupling Factor 1 (CF1) with dicyclohexylcarbodiimide (DCCD) results in the inactivation of the ATPase. The DCCD inactivation is time- and concentration-dependent. Complete inactivation of the CF1-ATPase activity requires the binding of 2 mol of DCCD/mol of CF1. The binding sites of DCCD are located on the beta subunit of CF1. 2. DCCD modification of soluble CF1 eliminates one adenine nucleotide binding site which is exposed by dithiothreitol activation or by incubation with tentoxin. The inactivation of both the ATPase activity and the adenine nucleotide binding site are pH-dependent. The inactivation of both the ATPase activity and the adenine nucleotide binding site are pH-dependent. Half-maximal inhibition occurs at about pH 7.5. 3. The DCCD-modified CF1, reconstituted with EDTA-treated chloroplasts, is fully active is restoring proton uptake but not in restoring ATP synthesis or light-dependent adenine nucleotide exchange. |
Authors:
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V Shoshan; B R Selman |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: The Journal of biological chemistry Volume: 255 ISSN: 0021-9258 ISO Abbreviation: J. Biol. Chem. Publication Date: 1980 Jan |
Date Detail:
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Created Date: 1980-03-24 Completed Date: 1980-03-24 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 2985121R Medline TA: J Biol Chem Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 384-9 Citation Subset: IM |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Adenosine Triphosphatases
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metabolism Adenylyl Imidodiphosphate / pharmacology Binding Sites Carbodiimides* Dicyclohexylcarbodiimide* Kinetics Macromolecular Substances Plants / enzymology Protein Binding Proton-Translocating ATPases* / metabolism |
| Chemical | |
Reg. No./Substance:
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0/Carbodiimides; 0/Macromolecular Substances; 25612-73-1/Adenylyl Imidodiphosphate; 538-75-0/Dicyclohexylcarbodiimide; EC 3.6.1.-/Adenosine Triphosphatases; EC 3.6.3.14/Proton-Translocating ATPases |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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