Document Detail

The interaction of C.I. acid red 27 with human hemoglobin in solution.
MedLine Citation:
PMID:  20638979     Owner:  NLM     Status:  MEDLINE    
The nature of the interaction between human hemoglobin and C.I. acid red 27 was investigated systematically by ultraviolet-vis absorbance, circular dichroism, fluorescence, synchronous fluorescence, and three-dimensional fluorescence spectra techniques at pH 7.40. The quenching mechanism, binding constants, and the number of binding sites were determined by the quenching of human hemoglobin fluorescence in presence of C.I. acid red 27. The results showed that the nature of the quenching was of static type and the process of binding acid red 27 on human hemoglobin was a spontaneous molecular interaction procedure. The electrostatic and hydrophobic interactions played a major role in stabilizing the complex; The distance r between donor and acceptor was obtained to be 4.40 nm according to Förster's theory; The effect of acid red 27 on the conformation of human hemoglobin was analyzed using synchronous fluorescence, circular dichroism and three-dimensional fluorescence spectra.
Yan-Qing Wang; Hong-Mei Zhang; Bo-Ping Tang
Related Documents :
2778809 - Leakage of heart fatty acid binding protein with ischemia and reperfusion in the rat.
6487649 - Fatty acid incorporation increases the affinity of muscarinic cholinergic receptors for...
11731339 - Binding properties of echinococcus granulosus fatty acid binding protein.
15518519 - Interactions of dendrimers with selected amino acids and proteins studied by continuous...
17007889 - Selectivity of fatty acid ligands for pparalpha which correlates both with binding to c...
2111739 - Protective role of intracoronary fatty acid binding protein in ischemic and reperfused ...
16945129 - Ethnopharmacological evaluation of radal (leaves of lomatia hirsuta) and isolation of 2...
23122099 - Salicylic acid-induced elicitation of folates in coriander (coriandrum sativum l.) impr...
8368319 - Cell survival in rabbit gastric glands: effect of extracellular ph, osmolarity, and ano...
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of photochemistry and photobiology. B, Biology     Volume:  100     ISSN:  1873-2682     ISO Abbreviation:  J. Photochem. Photobiol. B, Biol.     Publication Date:  2010 Aug 
Date Detail:
Created Date:  2010-07-19     Completed Date:  2010-10-14     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8804966     Medline TA:  J Photochem Photobiol B     Country:  Switzerland    
Other Details:
Languages:  eng     Pagination:  76-83     Citation Subset:  IM    
Copyright Information:
2010 Elsevier B.V. All rights reserved.
Jiangsu Provincial Key Laboratory of Coastal Wetland Bioresources and Environmental Protection, Yancheng City, Jiangsu Province, People's Republic of China.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Azo Compounds / chemistry*
Binding Sites
Circular Dichroism
Coloring Agents / chemistry*
Energy Transfer
Hemoglobins / chemistry*
Protein Binding
Spectrometry, Fluorescence
Spectrophotometry, Ultraviolet
Static Electricity
Reg. No./Substance:
0/Acid Red 27; 0/Azo Compounds; 0/Coloring Agents; 0/Hemoglobins; 0/Solutions

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  In vivo microscopy in a porcine model of acute lung injury.
Next Document:  Authors' Reply.