| The interaction of C.I. acid red 27 with human hemoglobin in solution. | |
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MedLine Citation:
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PMID: 20638979 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The nature of the interaction between human hemoglobin and C.I. acid red 27 was investigated systematically by ultraviolet-vis absorbance, circular dichroism, fluorescence, synchronous fluorescence, and three-dimensional fluorescence spectra techniques at pH 7.40. The quenching mechanism, binding constants, and the number of binding sites were determined by the quenching of human hemoglobin fluorescence in presence of C.I. acid red 27. The results showed that the nature of the quenching was of static type and the process of binding acid red 27 on human hemoglobin was a spontaneous molecular interaction procedure. The electrostatic and hydrophobic interactions played a major role in stabilizing the complex; The distance r between donor and acceptor was obtained to be 4.40 nm according to Förster's theory; The effect of acid red 27 on the conformation of human hemoglobin was analyzed using synchronous fluorescence, circular dichroism and three-dimensional fluorescence spectra. |
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Authors:
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Yan-Qing Wang; Hong-Mei Zhang; Bo-Ping Tang |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Journal of photochemistry and photobiology. B, Biology Volume: 100 ISSN: 1873-2682 ISO Abbreviation: J. Photochem. Photobiol. B, Biol. Publication Date: 2010 Aug |
Date Detail:
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Created Date: 2010-07-19 Completed Date: 2010-10-14 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 8804966 Medline TA: J Photochem Photobiol B Country: Switzerland |
Other Details:
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Languages: eng Pagination: 76-83 Citation Subset: IM |
Copyright Information:
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2010 Elsevier B.V. All rights reserved. |
Affiliation:
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Jiangsu Provincial Key Laboratory of Coastal Wetland Bioresources and Environmental Protection, Yancheng City, Jiangsu Province, People's Republic of China. wyqing76@126.com |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Azo Compounds
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chemistry* Binding Sites Circular Dichroism Coloring Agents / chemistry* Energy Transfer Hemoglobins / chemistry* Humans Hydrophobicity Protein Binding Solutions Spectrometry, Fluorescence Spectrophotometry, Ultraviolet Static Electricity |
| Chemical | |
Reg. No./Substance:
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0/Acid Red 27; 0/Azo Compounds; 0/Coloring Agents; 0/Hemoglobins; 0/Solutions |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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