Document Detail


The intellectual disability of trisomy 21: differences in gene expression in a case series of patients with lower and higher IQ.
MedLine Citation:
PMID:  23422941     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Trisomy 21 (T21), or Down syndrome (DS), is the most frequent and recognizable cause of intellectual disabilities. The level of disability, as evaluated by the intelligence quotient (IQ) test, varies considerably between patients independent of other factors. To determine the genetic or molecular basis of this difference, a high throughput transcriptomic analysis was performed on twenty T21 patients with high and low IQ, and 10 healthy controls using Digital Gene Expression. More than 90 millions of tags were sequenced in the three libraries. A total of 80 genes of potential interest were selected for the qPCR experiment validation, and three housekeeping genes were used for normalizing purposes. HLA DQA1 and HLA DRB1 were significantly downregulated among the patients with a low IQ, the values found in the healthy controls being intermediate between those noted in the IQ+ and IQ- T21 patients. Interestingly, the intergenic region between these genes contains a binding sequence for the CCCTC-binding factor, or CTCF, and cohesin (a multisubunit complex), both of which are essential for expression of HLA DQA1 and HLA DRB1 and numerous other genes. Our results might lead to the discovery of genes, or genetic markers, that are directly involved in several phenotypes of DS and, eventually, to the identification of potential targets for therapeutic interventions.
Authors:
André Mégarbané; Florian Noguier; Samantha Stora; Laurent Manchon; Clotilde Mircher; Roman Bruno; Nathalie Dorison; Fabien Pierrat; Marie-Odile Rethoré; Bernadette Trentin; Aimé Ravel; Marine Morent; Gerard Lefranc; David Piquemal
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2013-02-20
Journal Detail:
Title:  European journal of human genetics : EJHG     Volume:  21     ISSN:  1476-5438     ISO Abbreviation:  Eur. J. Hum. Genet.     Publication Date:  2013 Nov 
Date Detail:
Created Date:  2013-10-17     Completed Date:  2014-05-07     Revised Date:  2014-11-04    
Medline Journal Info:
Nlm Unique ID:  9302235     Medline TA:  Eur J Hum Genet     Country:  England    
Other Details:
Languages:  eng     Pagination:  1253-9     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Adolescent
Adult
Case-Control Studies
Down Syndrome / blood,  genetics*
Female
Gene Expression Profiling
Gene Expression Regulation*
HLA-DQ alpha-Chains / genetics
HLA-DRB1 Chains / genetics
Humans
Intellectual Disability / blood,  genetics*
Intelligence Tests*
Male
RNA, Messenger / genetics,  metabolism
Young Adult
Chemical
Reg. No./Substance:
0/HLA-DQ alpha-Chains; 0/HLA-DQA1 antigen; 0/HLA-DRB1 Chains; 0/RNA, Messenger
Comments/Corrections

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