Document Detail

An integrated scheme for the simultaneous determination of biogenic amines, precursor amino acids, and related metabolites by liquid chromatography with electrochemical detection.
MedLine Citation:
PMID:  6746835     Owner:  NLM     Status:  MEDLINE    
A new method using high-performance liquid chromatography with electrochemical detection (HPLC-ED) for the simultaneous determination of monoamines, their precursor amino acids, and related major metabolites in small samples of brain tissue weighing from 0.5 to 50 mg is described. The method is based on the preliminary isolation of monoamines (dopamine, norepinephrine, epinephrine, and serotonin), their precursor amino acids (tyrosine, 3,4-dihydroxyphenylalanine, tryptophan and 5-hydroxytryptophan), and their major metabolites (3-methoxytyramine, normetanephrine, 3,4-dihydroxyphenylacetic acid, homovanillic acid, vanillylmandelic acid, 3-methoxy-4-hydroxyphenylethyleneglycol, and 5-hydroxyindoleacetic acid) by chromatography on small columns of Amberlite CG-50 and Dowex 50W, and by ethyl acetate extraction. All the compounds in the four isolated fractions were measured by HPLC-ED on a reversed-phase column under four different conditions. The sensitivity was from 0.1 to 40 pmol, depending on the substances analysed. This newly established method was applied to the study of the effects of an aromatic L-amino acid decarboxylase inhibitor (NSD-1015) and a monoamine oxidase inhibitor (pargyline) on the levels of monoamines, their precursor amino acids and their major metabolites in brain regions of mice.
K Oka; K Kojima; A Togari; T Nagatsu; B Kiss
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of chromatography     Volume:  308     ISSN:  -     ISO Abbreviation:  J. Chromatogr.     Publication Date:  1984 Jun 
Date Detail:
Created Date:  1984-09-12     Completed Date:  1984-09-12     Revised Date:  2013-12-13    
Medline Journal Info:
Nlm Unique ID:  0427043     Medline TA:  J Chromatogr     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  43-53     Citation Subset:  IM    
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MeSH Terms
Amino Acids / analysis*,  metabolism
Aromatic-L-Amino-Acid Decarboxylases / antagonists & inhibitors
Biogenic Amines / analysis*,  metabolism
Brain Chemistry / drug effects
Chromatography, High Pressure Liquid / methods
Chromatography, Liquid / methods
Pargyline / pharmacology
Reg. No./Substance:
0/Amino Acids; 0/Biogenic Amines; 9MV14S8G3E/Pargyline; EC Decarboxylases

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