| An integrated optofluidic platform for Raman-activated cell sorting. | |
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MedLine Citation:
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PMID: 18584087 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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We report on integrated optofluidic Raman-activated cell sorting (RACS) platforms that combine multichannel microfluidic devices and laser tweezers Raman spectroscopy (LTRS) for delivery, identification, and simultaneous sorting of individual cells. The system allows label-free cell identification based on Raman spectroscopy and automated continuous cell sorting. Two optofluidic designs using hydrodynamic focusing and pinch-flow fractionation are evaluated based on their sorting design and flow velocity effect on the laser trapping efficiency at different laser power levels. A proof-of-principle demonstration of the integrated optofluidic LTRS system for the identification and sorting of two leukemia cell lines is presented. This functional prototype lays the foundation for the development of a label-free cell sorting platform based on intrinsic Raman markers for automated sampling and sorting of a large number of individual cells in solution. |
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Authors:
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Adrian Y Lau; Luke P Lee; James W Chan |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S. Date: 2008-05-28 |
Journal Detail:
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Title: Lab on a chip Volume: 8 ISSN: 1473-0197 ISO Abbreviation: Lab Chip Publication Date: 2008 Jul |
Date Detail:
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Created Date: 2008-06-27 Completed Date: 2008-09-12 Revised Date: 2008-11-21 |
Medline Journal Info:
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Nlm Unique ID: 101128948 Medline TA: Lab Chip Country: England |
Other Details:
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Languages: eng Pagination: 1116-20 Citation Subset: IM |
Affiliation:
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Applied Physics and Biophysics Division, Lawrence Livermore National Laboratory, P.O. Box 808, L-211, Livermore, CA 94550, USA. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Cell Separation
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instrumentation* Lymphocytes / cytology Microfluidic Analytical Techniques / instrumentation* Optics and Photonics* Sensitivity and Specificity Spectrum Analysis, Raman / instrumentation* |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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