| An integrated microfluidic system for rapid screening of alpha-fetoprotein-specific aptamers. | |
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MedLine Citation:
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PMID: 22410487 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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The systematic evolution of ligands by exponential enrichment (SELEX) is a screening technique that involves the progressive selection of highly specific ligands via repeated rounds of partition and amplification from a large random pool of nucleic acid sequences. The products of this selection process are called aptamers and are either short single-stranded deoxyribonucleic acid (ssDNA) or ribonucleic acid (RNA) molecules with a high binding affinity to a large variety of target analytes. However, SELEX is a lengthy, labor-intensive, iterative process requiring multiple rounds of extraction and polymerase chain reaction (PCR) amplification. In order to address these problems, this study presents a new integrated microfluidic system consisting of a magnetic bead-based microfluidic SELEX chip and a competitive assay chip to automate the aptamer screening process. More importantly, the selected ssDNA sequences were confirmed to have a high affinity and specificity to the target molecules, using the developed competitive assay chip. With this approach, an aptamer specific to alpha-fetoprotein (AFP), which is a biomarker for liver cancers, has been successfully selected. The screened aptamer was used as a recognition molecule for AFP and has a linear detection range from 12.5 to 800ng/mL, which was suitable for rapid clinical applications. |
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Authors:
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Chao-Jyun Huang; Hsin-I Lin; Shu-Chu Shiesh; Gwo-Bin Lee |
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Publication Detail:
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Type: JOURNAL ARTICLE Date: 2012-2-22 |
Journal Detail:
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Title: Biosensors & bioelectronics Volume: - ISSN: 1873-4235 ISO Abbreviation: - Publication Date: 2012 Feb |
Date Detail:
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Created Date: 2012-3-13 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9001289 Medline TA: Biosens Bioelectron Country: - |
Other Details:
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Languages: ENG Pagination: - Citation Subset: - |
Copyright Information:
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Copyright © 2012 Elsevier B.V. All rights reserved. |
Affiliation:
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Department of Engineering Science, National Cheng Kung University, Tainan 70101, Taiwan. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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