| An integrated in vitro and in vivo high-throughput screen identifies treatment leads for ependymoma. | |
| | |
MedLine Citation:
|
PMID: 21907928 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
Using a mouse model of ependymoma-a chemoresistant brain tumor-we combined multicell high-throughput screening (HTS), kinome-wide binding assays, and in vivo efficacy studies, to identify potential treatments with predicted toxicity against neural stem cells (NSC). We identified kinases within the insulin signaling pathway and centrosome cycle as regulators of ependymoma cell proliferation, and their corresponding inhibitors as potential therapies. FDA approved drugs not currently used to treat ependymoma were also identified that posses selective toxicity against ependymoma cells relative to normal NSCs both in vitro and in vivo, e.g., 5-fluorouracil. Our comprehensive approach advances understanding of the biology and treatment of ependymoma including the discovery of several treatment leads for immediate clinical translation. |
| | |
Authors:
|
Jennifer M Atkinson; Anang A Shelat; Angel Montero Carcaboso; Tanya A Kranenburg; Leggy A Arnold; Nidal Boulos; Karen Wright; Robert A Johnson; Helen Poppleton; Kumarasamypet M Mohankumar; Clementine Féau; Timothy Phoenix; Paul Gibson; Liqin Zhu; Yiai Tong; Chris Eden; David W Ellison; Waldemar Priebe; Dimpy Koul; W K Alfred Yung; Amar Gajjar; Clinton F Stewart; R Kiplin Guy; Richard J Gilbertson |
Related Documents
:
|
21517028 - Comparative cytotoxicity of gambierol versus other marine neurotoxins. 2155008 - Octamer motif mediates transcriptional repression of hsv immediate-early genes and octa... 2825168 - Cell-specific activity of the constituent elements of the simian virus 40 enhancer. 7948108 - Myc protein: partners and antagonists. 22737478 - The effect of fig tree latex (ficus carica) on stomach cancer line. 21304158 - How cells use pseudopods for persistent movement and navigation. |
Publication Detail:
|
Type: Journal Article; Research Support, N.I.H., Extramural |
Journal Detail:
|
Title: Cancer cell Volume: 20 ISSN: 1878-3686 ISO Abbreviation: Cancer Cell Publication Date: 2011 Sep |
Date Detail:
|
Created Date: 2011-09-12 Completed Date: 2011-11-07 Revised Date: 2012-09-28 |
Medline Journal Info:
|
Nlm Unique ID: 101130617 Medline TA: Cancer Cell Country: United States |
Other Details:
|
Languages: eng Pagination: 384-99 Citation Subset: IM |
Copyright Information:
|
Copyright © 2011 Elsevier Inc. All rights reserved. |
Affiliation:
|
Department of Developmental Neurobiology, St. Jude Children's Research Hospital, Memphis, TN 38105, USA. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Animals Boronic Acids / pharmacology Brain / pathology Brain Neoplasms / drug therapy*, metabolism, pathology Cell Proliferation Centrosome / metabolism Drug Screening Assays, Antitumor* Ependymoma / drug therapy*, metabolism, pathology Fluorouracil / pharmacology High-Throughput Screening Assays / methods* Insulin / metabolism Mice Mice, Nude Neural Stem Cells / drug effects* Pyrazines / pharmacology Signal Transduction Tumor Cells, Cultured |
| Grant Support | |
ID/Acronym/Agency:
|
P01CA96832/CA/NCI NIH HHS; P30CA021765/CA/NCI NIH HHS; R01 CA129541-01/CA/NCI NIH HHS; R01 CA129541-02/CA/NCI NIH HHS; R01 CA129541-03/CA/NCI NIH HHS; R01 CA129541-04/CA/NCI NIH HHS; R01 CA129541-05/CA/NCI NIH HHS; R01CA129541/CA/NCI NIH HHS |
| Chemical | |
Reg. No./Substance:
|
0/Boronic Acids; 0/Insulin; 0/Pyrazines; 0/bortezomib; 51-21-8/Fluorouracil |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Mesenchymal Stem Cells Induce Resistance to Chemotherapy through the Release of Platinum-Induced Fat...
Next Document: Exploiting cancer cell vulnerabilities to develop a combination therapy for ras-driven tumors.