Document Detail


The instability of (GpT)n and (ApC)n microsatellites induced by formaldehyde in Escherichia coli.
MedLine Citation:
PMID:  17630409     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Formaldehyde, a potential human nasal carcinogen, has been reported to induce DNA lesions. However, the effect of formaldehyde on microsatellite instability has not previously been reported. Plasmids containing different lengths of complementary (ApC)(n) or (GpT)(n) dinucleotide repeats on the leading strand were constructed to investigate whether the mutagenesis by formaldehyde can contribute to microsatellite instability. We observed that exposure of Escherichia coli to 2.5 mM formaldehyde increased the frequency of expansions and deletions of the dinucleotide repetitive sequences. After being induced by formaldehyde, the microsatellite mutation frequencies of (GpT)(n) and (ApC)(n) were 2- to 24-fold higher than those in the control. Although complementary to each other, (ApC)(n) and (GpT)(n) had different mutation frequencies when they were on the leading strand: mutation frequencies of (GpT)(n) were 13- to 24-fold higher than the control group, whereas frequencies of (ApC)(n) were only 2- to 3-fold higher the control group. Sequencing of the repetitive and flanking sequences in mutant clones showed that all mutants displayed expansions or deletions of dinucleotide repeats. These results clearly suggest that formaldehyde can increase microsatellite instability by affecting the fidelity of microsatellite maintenance. We presumed that a mutagenic mechanism of formaldehyde and the temporal formation of left-handed helix Z-DNA might be related to the microsatellite instability.
Authors:
Wei Wang; Jun Xu; Liu Xu; Bisong Yue; Fangdong Zou
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Publication Detail:
Type:  Journal Article     Date:  2007-07-14
Journal Detail:
Title:  Mutagenesis     Volume:  22     ISSN:  0267-8357     ISO Abbreviation:  Mutagenesis     Publication Date:  2007 Sep 
Date Detail:
Created Date:  2007-08-30     Completed Date:  2008-01-15     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8707812     Medline TA:  Mutagenesis     Country:  England    
Other Details:
Languages:  eng     Pagination:  353-7     Citation Subset:  IM    
Affiliation:
Key Laboratory of Bio-resources and Eco-environment (Ministry of Education), College of Life Sciences, Sichuan University, Chengdu 610064, People's Republic of China.
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MeSH Terms
Descriptor/Qualifier:
Dinucleotide Repeats / drug effects*
Escherichia coli / drug effects*,  genetics
Formaldehyde / pharmacology*
Microsatellite Instability*
Microsatellite Repeats / drug effects*
Plasmids / drug effects
Sequence Deletion
Chemical
Reg. No./Substance:
50-00-0/Formaldehyde

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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