Document Detail


An inositol polyphosphate 5-phosphatase functions in PHOTOTROPIN1 signaling in Arabidopis by altering cytosolic Ca2+.
MedLine Citation:
PMID:  18252844     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Inositol polyphosphate 5-phosphatase (5PTase) is a key enzyme in the phosphatidylinositol metabolic pathway, which plays critical roles in a number of cellular processes in plants. Our previous work implicated the role of 5PTase13, which encodes a WD40-containing type II 5PTase, in hormone-mediated cotyledon vein development. Here, we show that 5PTase13 is also involved in blue light responses in Arabidopsis thaliana. Compared with that in darkness, the expression of 5PTase13 was suppressed by blue light irradiation, and disruption of the gene resulted in shortened hypocotyls and expanded cotyledons. Genetic analysis showed that 5PTase13 acted independently from CRYPTOCHROME1 and CONSTITUTIVE PHOTOMORPHOGENIC1 but interacted functionally with PHOTOTROPIN1 (PHOT1). The expression level of 5PTase13 was significantly enhanced in phot1 single or phot1 phot2 double mutants under blue light, and suppression of 5PTase13 expression rescued the elongated hypocotyls in the phot1 or phot1 phot2 mutants. Further analysis showed that the blue light-induced elevation of cytosolic Ca2+ was inhibited in the phot1 mutant but enhanced in the 5pt13 mutant, suggesting that 5PTase13 antagonizes PHOT1-mediated effects on calcium signaling under blue light.
Authors:
Xu Chen; Wen-Hui Lin; Yuan Wang; Sheng Luan; Hong-Wei Xue
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2008-02-05
Journal Detail:
Title:  The Plant cell     Volume:  20     ISSN:  1040-4651     ISO Abbreviation:  Plant Cell     Publication Date:  2008 Feb 
Date Detail:
Created Date:  2008-04-01     Completed Date:  2008-07-31     Revised Date:  2013-06-06    
Medline Journal Info:
Nlm Unique ID:  9208688     Medline TA:  Plant Cell     Country:  United States    
Other Details:
Languages:  eng     Pagination:  353-66     Citation Subset:  IM    
Affiliation:
Shanghai Institutes for Biological Science-University of California Berkeley Center of Molecular Life Sciences, Chinese Academy of Sciences, 20032 Shanghai, China.
Data Bank Information
Bank Name/Acc. No.:
GENBANK/AJ297426; RefSeq/NM_103814;  NM_114447;  NM_116961;  NM_121018;  NM_125199;  NM_128855
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MeSH Terms
Descriptor/Qualifier:
Arabidopsis / genetics,  growth & development,  metabolism*
Arabidopsis Proteins / genetics,  metabolism*,  physiology
Calcium / metabolism*
Cotyledon / genetics,  growth & development,  metabolism
Cryptochromes
Cytosol / metabolism
Flavoproteins / genetics,  metabolism
Hypocotyl / genetics,  growth & development,  metabolism
Immunoprecipitation
Light
Molecular Sequence Data
Phosphoproteins / genetics,  metabolism,  physiology
Phosphoric Monoester Hydrolases / genetics,  metabolism*,  physiology
Plants, Genetically Modified / genetics,  growth & development,  metabolism
Protein Binding
Reverse Transcriptase Polymerase Chain Reaction
Signal Transduction / genetics,  physiology*
Two-Hybrid System Techniques
Chemical
Reg. No./Substance:
0/Arabidopsis Proteins; 0/CRY1 protein, Arabidopsis; 0/Cryptochromes; 0/Flavoproteins; 0/NPH1 protein, Arabidopsis; 0/PHOT2 protein, Arabidopsis; 0/Phosphoproteins; 0/constitutive photomorphogenic 1 protein, Arabidopsis; 7440-70-2/Calcium; EC 3.1.3.-/5PTase13 protein, Arabidopsis; EC 3.1.3.-/Phosphoric Monoester Hydrolases; EC 3.1.3.36/phosphoinositide 5-phosphatase
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