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The inner centromere protein (INCENP) antigens: movement from inner centromere to midbody during mitosis.
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MedLine Citation:
PMID:  3316246     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
We describe a novel set of polypeptide antigens that shows a dramatic change in structural localization during mitosis. Through metaphase these antigens define a new chromosomal substructure that is located between the sister chromatids. Because the antigens are concentrated in the pericentromeric region, we have provisionally termed them the INCENPs (inner centromere proteins). The INCENPs (two polypeptides of 155 and 135 kD) were identified with a monoclonal antibody that was raised against the bulk proteins of the mitotic chromosome scaffold fraction. These two polypeptides are the most tightly bound chromosomal proteins known. When scaffolds are prepared, 100% of the detectable INCENPs remain scaffold associated. We were therefore unprepared for the fate of the INCENPs at anaphase. As the sister chromatids separate, the INCENPs dissociate fully from them, remaining behind at the metaphase plate as the chromatids migrate to the spindle poles. During anaphase the INCENPs are found on coarse fibers in the central spindle, and also in close apposition to the cell membrane in the region of the forming contractile ring. During telophase, the INCENPs gradually become focused onto the forming midbody, together with which they are ultimately discarded. Several possible in vivo roles for the INCENPs are suggested by these data: regulation of sister chromatid pairing, stabilization of the plane of cleavage, and separation of spindle poles at anaphase.
Authors:
C A Cooke; M M Heck; W C Earnshaw
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, Non-P.H.S.; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  The Journal of cell biology     Volume:  105     ISSN:  0021-9525     ISO Abbreviation:  J. Cell Biol.     Publication Date:  1987 Nov 
Date Detail:
Created Date:  1988-01-07     Completed Date:  1988-01-07     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  0375356     Medline TA:  J Cell Biol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  2053-67     Citation Subset:  IM    
Affiliation:
Department of Cell Biology and Anatomy, Johns Hopkins School of Medicine, Baltimore, Maryland 21205.
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MeSH Terms
Descriptor/Qualifier:
Anaphase
Antibodies, Monoclonal / diagnostic use
Antigens, Nuclear
Cell Line
Centromere / ultrastructure*
Chromosomes / ultrastructure*
Fluorescent Antibody Technique
Metaphase
Mitosis
Nuclear Proteins / immunology,  physiology*
Grant Support
ID/Acronym/Agency:
GM30985/GM/NIGMS NIH HHS
Chemical
Reg. No./Substance:
0/Antibodies, Monoclonal; 0/Antigens, Nuclear; 0/Nuclear Proteins
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Full Text
Journal Information
Journal ID (nlm-ta): J Cell Biol
ISSN: 0021-9525
ISSN: 1540-8140
Publisher: The Rockefeller University Press
Article Information
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Print publication date: Day: 1 Month: 11 Year: 1987
Volume: 105 Issue: 5
First Page: 2053 Last Page: 2067
ID: 2114862
Publisher Id: 88059239
PubMed Id: 3316246

The inner centromere protein (INCENP) antigens: movement from inner centromere to midbody during mitosis


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