Document Detail


The inhibitory effects of deltamethrin on dopamine biosynthesis in rat PC12 cells.
MedLine Citation:
PMID:  16229977     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The inhibitory effects of deltamethrin (DM) on dopamine biosynthesis in PC12 cells were investigated. Levels of dopamine in PC12 cells were detected after treatment with different doses from 10(-4)M to 10(-6)M deltamethrin or combined with 20 microM or 50 microM l-3,4-dihydroxyphenylalanine (L-Dopa) for 3 h or 12 h by HPLC. Flow cytometry (FCM) and RT-PCR technology were used to detect the levels of tyrosine hydroxylase (TH) mRNA and protein in PC12 cells treated with different doses of deltamethrin for 3 h or 12 h. Deltamethrin decreased the levels of dopamine, TH mRNA and TH protein in PC12 cells, and the increased dopamine levels induced by l-Dopa at 20-50 microM were also decreased by deltamethrin for 3 h or 12 h. These results indicate that deltamethrin decreases dopamine content by the inhibition of TH mRNA and protein level in PC12 cells and maybe also have inhibitory effect on aromatic l-amino acid decarboxylase.
Authors:
Gong-Ping Liu; Nian Shi
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Publication Detail:
Type:  Journal Article     Date:  2005-10-17
Journal Detail:
Title:  Toxicology letters     Volume:  161     ISSN:  0378-4274     ISO Abbreviation:  Toxicol. Lett.     Publication Date:  2006 Mar 
Date Detail:
Created Date:  2006-01-27     Completed Date:  2006-03-16     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  7709027     Medline TA:  Toxicol Lett     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  195-9     Citation Subset:  IM    
Affiliation:
Department of Toxicology, MOE Key Laboratory of Environment and Health, School of Public Health, Tongji Medical College of Huazhong Science and Technology University, Wuhan 430030, China.
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MeSH Terms
Descriptor/Qualifier:
Animals
Dopamine / biosynthesis*
Dose-Response Relationship, Drug
Drug Combinations
Enzyme Inhibitors / toxicity*
Gene Expression / drug effects
Insecticides / toxicity*
Levodopa / pharmacology
Nitriles / toxicity*
PC12 Cells / drug effects*,  metabolism
Pyrethrins / toxicity*
RNA, Messenger / analysis
Rats
Tyrosine 3-Monooxygenase / antagonists & inhibitors,  genetics,  metabolism
Chemical
Reg. No./Substance:
0/Drug Combinations; 0/Enzyme Inhibitors; 0/Insecticides; 0/Levodopa; 0/Nitriles; 0/Pyrethrins; 0/RNA, Messenger; 52820-00-5/decamethrin; EC 1.14.16.2/Tyrosine 3-Monooxygenase

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