Document Detail


The inhibitory effect of whole and deproteinized saliva on mutagenicity and clastogenicity resulting from a model nitrosation reaction.
MedLine Citation:
PMID:  6750391     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The objective of this study was to simulate in vitro at least some of the conditions that prevail in man during ingestion of nitrate and nitrosable compounds. Human saliva has been chosen because most chemicals ingested through food will interact with saliva. The nitrosation of methylurea was used as a model because the nitrosation products can be readily detected by their mutagenic (his+ revertants of S. typhimurium) and clastogenic (chromosome aberrations in CHO cells) properties. The results show that human saliva inhibits the formation of mutagenic and clastogenic nitrosation products when present during nitrosation. A 50% inhibition of mutagenicity results from the addition of a saliva sample diluted at 5% of the original concentration. In the test system used a similar inhibitory effect was obtained by 2.5 mM ascorbic acid or 2.0 mM chlorogenic acid. The main inhibitory agents seem to reside in a deproteinized fraction which was filtered through an ultrafilter UM2 (greater than 1000 MW). At strong acid levels (below pH 2) the saliva loses its inhibitory effect on the nitrosation of methylurea. The contribution of saliva to the inhibition of endogenous nitrosation within the oral cavity or stomach is discussed.
Authors:
H F Stich; M P Rosin; L Bryson
Publication Detail:
Type:  In Vitro; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Mutation research     Volume:  97     ISSN:  0027-5107     ISO Abbreviation:  Mutat. Res.     Publication Date:  1982 Aug 
Date Detail:
Created Date:  1982-12-02     Completed Date:  1982-12-02     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0400763     Medline TA:  Mutat Res     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  283-92     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Biotransformation
Cell Survival / drug effects
Female
Humans
Kinetics
Male
Methylurea Compounds / metabolism*
Mutagenicity Tests
Mutagens* / metabolism
Nitrites / metabolism*
Nitroso Compounds / metabolism*,  pharmacology
Saliva / metabolism*
Salmonella typhimurium / drug effects
Sodium Nitrite / metabolism*
Chemical
Reg. No./Substance:
0/Methylurea Compounds; 0/Mutagens; 0/Nitrites; 0/Nitroso Compounds; 598-50-5/methylurea; 7632-00-0/Sodium Nitrite

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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