Document Detail

The inhibitory effect of superparamagnetic iron oxide nanoparticle (Ferucarbotran) on osteogenic differentiation and its signaling mechanism in human mesenchymal stem cells.
MedLine Citation:
PMID:  20338187     Owner:  NLM     Status:  MEDLINE    
Superparamagnetic iron oxide (SPIO) nanoparticles are very useful for monitoring cell trafficking in vivo and distinguish whether cellular regeneration originated from an exogenous cell source, which is a key issue for developing successful stem cell therapies. However, the impact of SPIO labeling on stem cell behavior remains uncertain. Here, we show the inhibitory effect of Ferucarbotran, an ionic SPIO, on osteogenic differentiation and its signaling mechanism in human mesenchymal stem cells. Ferucarbotran caused a dose-dependent inhibition of osteogenic differentiation, abolished the differentiation at high concentration, promoted cell migration, and activated the signaling molecules, beta-catenin, a cancer/testis antigen, SSX, and matrix metalloproteinase 2 (MMP2). An iron chelator, desferrioxamine, suppressed all the above Ferucarbotran-induced actions, demonstrating an important role of free iron in the inhibition of osteogenic differentiation that is mediated by the promotion of cell mobilization, involving the activation of a specific signaling pathway.
Ying-Chun Chen; Jong-Kai Hsiao; Hon-Man Liu; I-Yin Lai; Ming Yao; Szu-Chun Hsu; Bor-Sheng Ko; Yao-Chang Chen; Chung-Shi Yang; Dong-Ming Huang
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Publication Detail:
Type:  In Vitro; Journal Article; Research Support, Non-U.S. Gov't     Date:  2010-03-22
Journal Detail:
Title:  Toxicology and applied pharmacology     Volume:  245     ISSN:  1096-0333     ISO Abbreviation:  Toxicol. Appl. Pharmacol.     Publication Date:  2010 Jun 
Date Detail:
Created Date:  2010-05-17     Completed Date:  2010-06-11     Revised Date:  2013-04-11    
Medline Journal Info:
Nlm Unique ID:  0416575     Medline TA:  Toxicol Appl Pharmacol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  272-9     Citation Subset:  IM    
Copyright Information:
(c) 2010 Elsevier Inc. All rights reserved.
Center for Nanomedicine Research, National Health Research Institutes, Zhunan, Miaoli County 350, Taiwan.
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MeSH Terms
Cell Differentiation / drug effects
Cell Movement / drug effects
Dose-Response Relationship, Drug
Ferumoxytol / toxicity*
Magnetite Nanoparticles
Matrix Metalloproteinase 2 / metabolism
Mesenchymal Stromal Cells / cytology,  drug effects*,  metabolism
Metal Nanoparticles
Osteogenesis / drug effects*
Signal Transduction
beta Catenin / metabolism
Reg. No./Substance:
0/Magnetite Nanoparticles; 0/beta Catenin; 1317-61-9/Ferumoxytol; 9004-54-0/Dextrans; EC Metalloproteinase 2; G6N3J05W84/ferumoxides

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