Document Detail


The inhibitory effect of heparin for vascular smooth muscle cell proliferation or migration is not mediated by u-PA and t-PA.
MedLine Citation:
PMID:  9187019     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Previous works suggest the interesting possibility of an effect of heparin on vascular smooth muscle cell (SMC) replication and migration via a selective inhibition of the expression of t-PA and u-PA both of which may play major roles during intimal hyperplasia following endothelial injury. The present study was undertaken to evaluate in vitro the effect of heparin on the growth and migration of aortic SMC isolated from transgenic mice showing single inactivations of the t-PA and u-PA genes comparatively to SMC isolated from control mice. With regard to serum-induced proliferation and migration, all cell types showed similar responses. On control cells, heparin inhibited in a dose-dependent manner the expression of both t-PA and u-PA protein and mRNA. Heparin however, similarly affected the mitogenic and chemotactic activity of FCS for SMC isolated from control, t-PA or u-PA-deficient mice therefore showing that heparin inhibits FCS-induced SMC proliferation via mechanism(s) other than single inhibition of t-PA or u-PA expression by smooth muscle cells.
Authors:
J M Herbert; F Bono; I Lamarche; P Carmeliet
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Thrombosis research     Volume:  86     ISSN:  0049-3848     ISO Abbreviation:  Thromb. Res.     Publication Date:  1997 May 
Date Detail:
Created Date:  1997-07-30     Completed Date:  1997-07-30     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  0326377     Medline TA:  Thromb Res     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  317-24     Citation Subset:  IM    
Affiliation:
Haemobiology Research Department, Sanofi Recherche, Toulouse, France. jean-marc.herbert@tls1.elfsanofi.fr
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MeSH Terms
Descriptor/Qualifier:
Animals
Aorta
Cell Division / drug effects
Cell Movement / drug effects*
Drug Interactions
Fetal Blood / physiology
Heparin / pharmacology*
Mice
Mice, Transgenic
Muscle, Smooth, Vascular / cytology,  drug effects*,  enzymology*
Tissue Plasminogen Activator / biosynthesis,  drug effects*,  genetics
Urokinase-Type Plasminogen Activator / biosynthesis,  drug effects*,  genetics
Chemical
Reg. No./Substance:
9005-49-6/Heparin; EC 3.4.21.68/Tissue Plasminogen Activator; EC 3.4.21.73/Urokinase-Type Plasminogen Activator

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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