| The inhibitory action of buformin, a biguanide on gluconeogenesis from alanine and its transport system in rat livers. | |
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MedLine Citation:
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PMID: 8472619 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The effect of buformin, a biguanide, on gluconeogenesis from 10 mM alanine in the presence of 143 nM glucagon were studied using isolated rat liver perfusions. In addition, to investigate possible mechanisms of biguanide action, alanine utilization in isolated rat liver perfusion and [3H]alanine uptake in isolated hepatocytes were observed. Buformin (1.85 mM) strongly inhibited gluconeogenesis from alanine in the presence of glucagon in both normal and streptozocin-induced diabetic rat livers. This inhibition was followed by a decrease in alanine utilization. Both of these inhibitory effects of buformin were dose-dependent. [3H]Alanine uptake was significantly inhibited by buformin. The effect of this agent was similar to but weaker than that of ouabain. However, tolbutamide failed to reduce either alanine utilization or [3H]alanine uptake, although this drug significantly inhibited gluconeogenesis from alanine. These data suggest that biguanides may reduce hepatic alanine utilization via the inhibition of Na+/L-alanine transport activity as one possible mechanism, resulting the inhibition of gluconeogenesis from alanine in the presence of glucagon. |
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Authors:
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N Hotta; T Komori; M Kobayashi; F Sakakibara; N Koh; N Sakamoto |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Diabetes research and clinical practice Volume: 19 ISSN: 0168-8227 ISO Abbreviation: Diabetes Res. Clin. Pract. Publication Date: 1993 Jan |
Date Detail:
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Created Date: 1993-05-17 Completed Date: 1993-05-17 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 8508335 Medline TA: Diabetes Res Clin Pract Country: NETHERLANDS |
Other Details:
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Languages: eng Pagination: 49-58 Citation Subset: IM |
Affiliation:
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Third Department of Internal Medicine, Nagoya University School of Medicine, Japan. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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3-Hydroxybutyric Acid Acetoacetates / metabolism Alanine / drug effects, pharmacokinetics* Animals Biological Transport, Active / drug effects Buformin / pharmacology* Cell Separation Diabetes Mellitus, Experimental / metabolism Glucagon / pharmacology Gluconeogenesis / drug effects* Hydroxybutyrates / metabolism Lactates / metabolism Lactic Acid Liver / drug effects*, metabolism Male Perfusion Pyruvates / metabolism Pyruvic Acid Rats Rats, Wistar Tolbutamide / pharmacology |
| Chemical | |
Reg. No./Substance:
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0/Acetoacetates; 0/Hydroxybutyrates; 0/Lactates; 0/Pyruvates; 127-17-3/Pyruvic Acid; 300-85-6/3-Hydroxybutyric Acid; 50-21-5/Lactic Acid; 541-50-4/acetoacetic acid; 56-41-7/Alanine; 64-77-7/Tolbutamide; 692-13-7/Buformin; 9007-92-5/Glucagon |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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