Document Detail

The inhibition of high and low molecular weight urokinase in plasma.
MedLine Citation:
PMID:  6444529     Owner:  NLM     Status:  MEDLINE    
The rates of inhibition of high molecular weight (HMW) and low molecular weight (LMW) urokinase (UK) incubated in plasma or with purified antithrombin III (AT-III) were compared. Using a fibrinolytic assay system to determine residual biologic activity, polyacrylamide gel electrophoresis to demonstrate the formation of complexes, and selective immunoprecipitation techniques to identify the plasma inhibitors participating in the neutralization process, it was established that: (A) HMW-UK is inhibited more rapidly than LMW-UK, both in plasma and with purified AT-III; (B) heparin (3--10 U/ml accelerates the neutralization process in both systems, but only slightly; and (C) in plasma, several inhibitors, alpha 2-macroglobulin, alpha 1-antitrypsin, and antithrombin III, neutralize the activity of HMW-UK and LMW-UK.
G Murano; D Aronson; L Williams; L Brown
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Blood     Volume:  55     ISSN:  0006-4971     ISO Abbreviation:  Blood     Publication Date:  1980 Mar 
Date Detail:
Created Date:  1980-05-14     Completed Date:  1980-05-14     Revised Date:  2009-11-19    
Medline Journal Info:
Nlm Unique ID:  7603509     Medline TA:  Blood     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  430-6     Citation Subset:  AIM; IM    
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MeSH Terms
Antithrombin III
Chromatography, Gel
Endopeptidases / blood*
Fibrinolysin / pharmacology
Heparin / pharmacology
Immunosorbent Techniques
Molecular Weight
Urokinase-Type Plasminogen Activator / antagonists & inhibitors,  blood*
Reg. No./Substance:
9000-94-6/Antithrombin III; 9001-91-6/Plasminogen; 9005-49-6/Heparin; EC 3.4.-/Endopeptidases; EC; EC Plasminogen Activator

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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