Document Detail


The inhibition of high and low molecular weight urokinase in plasma.
MedLine Citation:
PMID:  6444529     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The rates of inhibition of high molecular weight (HMW) and low molecular weight (LMW) urokinase (UK) incubated in plasma or with purified antithrombin III (AT-III) were compared. Using a fibrinolytic assay system to determine residual biologic activity, polyacrylamide gel electrophoresis to demonstrate the formation of complexes, and selective immunoprecipitation techniques to identify the plasma inhibitors participating in the neutralization process, it was established that: (A) HMW-UK is inhibited more rapidly than LMW-UK, both in plasma and with purified AT-III; (B) heparin (3--10 U/ml accelerates the neutralization process in both systems, but only slightly; and (C) in plasma, several inhibitors, alpha 2-macroglobulin, alpha 1-antitrypsin, and antithrombin III, neutralize the activity of HMW-UK and LMW-UK.
Authors:
G Murano; D Aronson; L Williams; L Brown
Related Documents :
7511169 - Binding of the protease-sensitive form of prp (prion protein) to sulfated glycosaminogl...
1730729 - Conversion of antithrombin from an inhibitor of thrombin to a substrate with reduced he...
2179409 - Immunologic and physicochemical evidence for conformational changes occurring on conver...
11915889 - Paraffinic hydrocarbons in the orgueil, murray, mokoia and other meteorites.
12717719 - Conformational behavior of chondroitin and chondroitin sulfate in relation to their phy...
17875649 - Mechanism by which exosites promote the inhibition of blood coagulation proteases by he...
24247249 - Groel/groes accelerates the refolding of a multi-domain protein through modulating on p...
19656819 - {alpha}' subunit of soybean {beta}-conglycinin forms complex with rice glutelin via a d...
22154939 - Inhibition of pseudomonas aeruginosa virulence: characterization of the apra-apri inter...
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Blood     Volume:  55     ISSN:  0006-4971     ISO Abbreviation:  Blood     Publication Date:  1980 Mar 
Date Detail:
Created Date:  1980-05-14     Completed Date:  1980-05-14     Revised Date:  2009-11-19    
Medline Journal Info:
Nlm Unique ID:  7603509     Medline TA:  Blood     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  430-6     Citation Subset:  AIM; IM    
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Animals
Antithrombin III
Chromatography, Gel
Endopeptidases / blood*
Fibrinolysin / pharmacology
Goats
Heparin / pharmacology
Humans
Immunosorbent Techniques
Molecular Weight
Plasminogen
Urokinase-Type Plasminogen Activator / antagonists & inhibitors,  blood*
Chemical
Reg. No./Substance:
9000-94-6/Antithrombin III; 9001-91-6/Plasminogen; 9005-49-6/Heparin; EC 3.4.-/Endopeptidases; EC 3.4.21.7/Fibrinolysin; EC 3.4.21.73/Urokinase-Type Plasminogen Activator

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Nicotinamide-induced hepatic microsomal mixed function oxidase system in rats.
Next Document:  The molecular mechanism of the inherited phosphofructokinase deficiency associated with hemolysis an...