| The inhibition of glioma growth in vitro and in vivo by a chitosan/ellagic acid composite biomaterial. | |
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MedLine Citation:
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PMID: 19501395 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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This study has developed a chitosan-based delivery system to locally administer ellagic acid for brain cancer treatment. We fabricated chitosan/ellagic acid composite films with various concentrations of ellagic acid. In vitro release study was performed by using a UV spectrophotometer, and enzymatic degradation rate was determined by analyzing the increased free amino groups. Viability of brain cancer cells (human U87 glioblastomas and rat C6 glioma cells) was measured via direct and indirect cell culture on the films by MTS assay. Caspase-3 activation, Western blot for p53, and anti-angiogenesis assays were also examined. In the in vivo study, GFP-tagged rat C6 glioma cells were implanted subcutaneously at the right flank region of nude mice and treatments were initiated by implanting the films subcutaneously. Tumor growth was evaluated by measuring tumor volume using a caliper, an ultrasound machine, and an optical imaging system. The chitosan/ellagic acid composite films were enzymatically degradable and exhibited a sustained slow release of ellagic acid. These materials could inhibit the cancer cell growth in an ellagic acid concentration-dependent manner by inducing apoptosis of cancer cells as well as suppressing angiogenesis. These materials also significantly suppressed tumor tissue growth in vivo. |
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Authors:
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Sungwoo Kim; Mostafa W Gaber; Janice A Zawaski; Feng Zhang; Mekel Richardson; Xin A Zhang; Yunzhi Yang |
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Publication Detail:
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Type: Journal Article Date: 2009-06-05 |
Journal Detail:
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Title: Biomaterials Volume: 30 ISSN: 1878-5905 ISO Abbreviation: Biomaterials Publication Date: 2009 Sep |
Date Detail:
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Created Date: 2009-08-03 Completed Date: 2009-11-10 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 8100316 Medline TA: Biomaterials Country: England |
Other Details:
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Languages: eng Pagination: 4743-51 Citation Subset: IM |
Affiliation:
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School of Biomedical Engineering and Imaging, University of Tennessee Health Science Center, Memphis, TN 31863, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Biocompatible Materials / pharmacology* Blotting, Western Caspase 3 / metabolism Cell Line, Tumor Cell Proliferation / drug effects Cell Survival / drug effects Chitosan / pharmacology* Drug Screening Assays, Antitumor Ellagic Acid / pharmacology* Enzyme Activation / drug effects Female Glioma / enzymology, pathology*, therapy Humans Mice Mice, Nude Muramidase / metabolism Neovascularization, Physiologic / drug effects Rats Tumor Suppressor Protein p53 / metabolism |
| Chemical | |
Reg. No./Substance:
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0/Biocompatible Materials; 0/Tumor Suppressor Protein p53; 476-66-4/Ellagic Acid; 9012-76-4/Chitosan; EC 3.2.1.17/Muramidase; EC 3.4.22.-/Caspase 3 |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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