| The inhibition of fluorescence resonance energy transfer between quantum dots for glucose assay. | |
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MedLine Citation:
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PMID: 22192453 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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Fluorescence resonance energy transfer (FRET) between two quantum dots of different sizes causes fluorescence quenching. Hereby a binding site pre-blocking approach is proposed to avoid this effect. Pre-binding of glucose on the donor occupies the binding sites and thus blocks resonance energy transfer between the two quantum dots, protecting the fluorescence from being quenched. A glucose assay is developed based on this approach. The glucose content is correlated with the fluorescence difference in the absence and in the presence of glucose. In practice, Green QDs-Con A conjugates are used as donors and Red QDs-NH(2)-glu conjugates as acceptors to form FRET system. The inhibition of fluorescence quenching is then measured in the presence of glucose. A linear calibration graph is achieved within 0.1-2.0mmolL(-1), along with a detection limit of 0.03mmolL(-1) and a RSD of 2.1% (1.0mmolL(-1)). 91-105% of glucose in serum and urine samples is recovered. It is worth mentioning that the present glucose assay approach also generates a fluorescence chromatic difference imaging, and the color display clearly identifies the glucose contents by visual detection with a distinguishing ability of ca. 0.5mmolL(-1). The present approach can potentially be used for the clinical determination of glucose in biological samples which can be further developed into a glucose sensor. |
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Authors:
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Bo Hu; Li-Pei Zhang; Mei-Ling Chen; Ming-Li Chen; Jian-Hua Wang |
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Publication Detail:
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Type: JOURNAL ARTICLE Date: 2011-11-30 |
Journal Detail:
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Title: Biosensors & bioelectronics Volume: - ISSN: 1873-4235 ISO Abbreviation: - Publication Date: 2011 Nov |
Date Detail:
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Created Date: 2011-12-23 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9001289 Medline TA: Biosens Bioelectron Country: - |
Other Details:
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Languages: ENG Pagination: - Citation Subset: - |
Copyright Information:
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Copyright © 2011 Elsevier B.V. All rights reserved. |
Affiliation:
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Research Center for Analytical Sciences, Northeastern University, Box 332, Shenyang 110819, China. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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