Document Detail


The inhibition of collagenase induced degradation of collagen by the galloyl-containing polyphenols tannic acid, epigallocatechin gallate and epicatechin gallate.
MedLine Citation:
PMID:  20162329     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Collagen based cosmetic fillers require repeat treatments due to collagenase derived degradation of the filler in the intradermal injection site. The objective of this study was to investigate the inhibition of this degradation by the galloyl-containing compounds tannic acid, epigallocatechin gallate (EGCG), epicatechin gallate (ECG) and gallic acid (GA). A gel permeation chromatography assay was developed to quantitate the collagenase induced reductions in collagen molecular weight. The binding of the compounds to collagen was measured using HPLC. The stabilization of collagen was measured using Differential Scanning Calorimetry (DSC). Tannic acid, EGCG and ECG (but not GA) were found to strongly inhibit collagen degradation at concentrations in the low micromolar range. The compounds bound strongly to collagen and stabilized collagen. It is concluded that tannic acid, EGCG and ECG bind to collagen via extensive hydrogen bonding augmented by some hydrophobic interactions and prevent the free access of collagenase to active sites on the collagen chains.
Authors:
John K Jackson; Jinying Zhao; Wesley Wong; Helen M Burt
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2010-02-17
Journal Detail:
Title:  Journal of materials science. Materials in medicine     Volume:  21     ISSN:  1573-4838     ISO Abbreviation:  J Mater Sci Mater Med     Publication Date:  2010 May 
Date Detail:
Created Date:  2010-05-12     Completed Date:  2010-09-10     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9013087     Medline TA:  J Mater Sci Mater Med     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1435-43     Citation Subset:  IM    
Affiliation:
Faculty of Pharmaceutical Sciences, University of British Columbia, Vancouver, BC, V6T 1Z3, Canada. burt@interchange.ubc.ca
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MeSH Terms
Descriptor/Qualifier:
Antioxidants / metabolism,  pharmacology*
Catechin / analogs & derivatives,  chemistry,  metabolism,  pharmacology
Collagen / metabolism
Collagenases / antagonists & inhibitors*,  metabolism
Extracellular Matrix / metabolism
Flavonoids
Gallic Acid / metabolism,  pharmacology
Phenols
Tannins / metabolism
Chemical
Reg. No./Substance:
0/Antioxidants; 0/Flavonoids; 0/Phenols; 0/Tannins; 0/polyphenols; 149-91-7/Gallic Acid; 154-23-4/Catechin; 863-03-6/epicatechin gallate; 9007-34-5/Collagen; 989-51-5/epigallocatechin gallate; EC 3.4.24.-/Collagenases

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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